Research Article Volume 20 Issue 3 - 2025

Nutritional, Hematological and Biochemical Quality of Albino Rat Fed on Complementary Food Produced from Acha (Digitaria exilis), Malted Pigeon Pea (Cajanus cajan), Grasshopper (Zonocerus variegatus) and Beetroot (Beta vulgaris L.) Flour Blends

Ayo Jerome Adekunle1*, Ibrahim Jummai2, Anikweze GU2, Paul Tailem3*, Shuaibu Zuwaira2, Shehu Umar1 and Ayele Esther Luba2

1Department of Food Science and Technology, Federal University Wukari, Wukari, Nigeria
2Department of Home Sciences and Magt., Nasarawa State University, Keffi (Shabu Lafia Campus), Nigeria
3Department of Home Economics, Adamu Tafawa Balewa College of Education Kangere Bauchi, Nigeria

*Corresponding Author: Ayo Jerome Adekunle, Department of Food Science and Technology, Federal University Wukari, Wukari, Nigeria and Paul Tailem, Department of Home Economics, Adamu Tafawa Balewa College of Education Kangere Bauchi, Nigeria.
Received: October 06, 2025; Published: November 03, 2025



Complementary feeding is the introduction of other foods (solid and liquids) when breast milk alone is no longer sufficient to support the nutritional requirements for the infant. This study evaluated the nutritional and hematological quality of albino rat fed on complementary foods produced from acha, malted pigeon pea, grasshopper, and beetroot flours blends at varied ratios (100:0:0:0; 85:5:5:5;75:15:5:5; 80.10:5:5;75::15:5:5 and 70:5:20:5 respectively); Results shows that the incorporation of these ingredients significantly enhanced Wistar rats growth performance 11.40 to 13.94%, nitrogen retention 0.97 to 2.12%, biological value 37.09 to 78.07%, supported by superior protein and carbohydrate digestibility. The WBC 4.45 to 7.30 (×10⁹/L), RBC 5.80 to 6.75 (×10¹²/L), HB, PVC, MCV, MCH and MCHC of the blood of rat fed with produced complimentary food showed significant (p < 0.05) differences in all the samples. Biochemical indices TP, albumin, Na⁺, K⁺, TC mdl creatinine and urea values ranged 5.52 to 6.30 g/L, 3.07 to 3.45 g/L, 144.15 to 150.85 mEq/L, 4.41 to 5.55 mmol/L, 113.60 to 130.95 TCmdl, and 38.38 to 41.50 mg/dL, 0.58 to 0.65 mg/dL respectively with added malted pigeon pea. These findings suggest that the blends, particularly F3 and F6 (Acha75%+Malted Pigeon pea15%+Grasshopper5%+Betroot5% and Acha70%+Malted Pigeon pea5%+Grasshopper20%+Betroot5%) are nutritionally adequate, safe, and highly acceptable complementary foods with strong potential to address protein-energy malnutrition and micronutrient deficiencies in infants. The study recommends further optimization, vitamin fortification, and strict hygienic processing to enhance the quality and scalability of these complementary foods.

 Keywords: Nutritional; Haematological Quality; Albino Rat; Complementary Food

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Ayo Jerome Adekunle., et al. “Nutritional, Hematological and Biochemical Quality of Albino Rat Fed on Complementary Food Produced from Acha (Digitaria exilis), Malted Pigeon Pea (Cajanus cajan), Grasshopper (Zonocerus variegatus) and Beetroot (Beta vulgaris L.) Flour Blends”. EC Nutrition  20.3 (2025): 01-17.