EC Nutrition

Research Article Volume 10 Issue 5 - 2017

Studies on the Nutritive Value, Antinutritional Factors and In Vitro Protein Digestibility of Maize Based Food Blend Fortified with Cowpea Bambaranut

Bintu BP, Hauwa H, Falmata AS, Modu S*, Aliyu Daja and Maryam BK

Departments of Biochemistry and Biological Sciences, Faculty of Science, University of Maiduguri, Maiduguri, Nigeria
*Corresponding Author: Modu Sherrif, Departments of Biochemistry and Biological Sciences, Faculty of Science, University of Maiduguri, Maiduguri, Nigeria.
Received: August 18, 2017; Published: September 11, 2017



The complementary weaning food blends were formulated using cereal- legume combination using yellow maize, cowpea, bam- baranut and groundnut. Yellow maize was fermented to produce “Akamu”, cowpea, bambaranut and groundnut were roasted. The weaning food blends were formulated as follows: MCBG: 60 (g) yellow maize: 20 (g) cowpea: 10 (g) bambaranut: 10 (g) groundnut, MCB; 60 (g) yellow maize: 20 (g) cowpea: 20 (g) bambaranut, MCG: 60 (g) yellow maize: 20 (g) bambaranut: 20 (g) groundnut and MBG: 60 (g) yellow maize: 20 (g) bambaranut: 20 (g) groundnut. The parameters assayed include proximate composition, mineral element, vitamin content, in vitro protein digestibility, anti-nutritional content, function properties, amino acid composition and sensory evaluation using standard laboratory methods. The yellow maize (Improved variety),cowpea, bambaranut and groundnut were obtained from Lake Chad Research Institute (LCRI) Maiduguri. The weaning food blends exhibited a low moisture content MCBG (3.37 ± 0.11%), MCB (3.38 ± 0.57%), MCG (3.43 ± 0.17%) and MBG (3.60 ± 0.20%). Protein content of MCBG (12.35 ± 0.06%) was close to the commercial weaning foods Cerelac® and Frisogold® and met the RDA of infants 0 - 1 year while the protein content of MCB (9.87 ± 0.06%), MCG (4.20 ± 0.04%) and MBG (3.61 ± 0.18%) were not comparable to commercial weaning foods Cerelac® and Frisogold® and have not met the RDA of infants 0 - 1 year, MCB was close to MCBG in the level of protein. The fat content of MCBG (9.58 ± 0.02%) met the RDA (10.25%) of infants 0 - 1 year and was comparable to the commercial weaning foods Cerelac® (10.00%) and Frisogold ® (7.30%), but that of MBG (6.80 ± 0.26%) was below the RDA and Cerelac®. MCB (2.94 ± 0.01%) and MCG (5.87 ± 0.32%) were below the RDA of infants 0 - 1 year and the commercial weaning foods Cerelac® and Frisogold® . The energy content of MCBG (403.00 ± 0.58 kcal) was comparable to the weaning foods Cerelac® (410.00 ± kcal/100g) and Frisogold® (415.00 kcal/100g) and met the RDA (400.00 kcal/100g) of infants 0 - 1 year. The levels of sodium, potassium, calcium and phosphorus of the weaning food blends MCBG, MCB, MCG and MBG were below the RDA of infants 0 - 1 year and were below the commercial weaning foods Cerelac ® and Frisogold® while the levels of magnesium, iron and zinc of the four weaning food blends met the RDA of infants and were comparable to the commercial weaning foods Cerelac® and Frisogold®. Increases were observed in the thiamine, Riboflavin, pyridox- ine, cobalamine of the weaning food blends MCBG, MCB, MCG and MBG met the RDA of infants 0 - 1 year while the Vitamin A and C content of the four weaning food blends were below the RDA and also below the commercial weaning foods Cerelac® and Frisogold® . Processing of the grains resulted in significant percentage reduction in phytic acid, yellow maize (74.4%) cowpea (62.5%), bambara- nut (50.2%), groundnut (70.0%) and reduction the tannin content yellow maize (60.7%), cowpea (71.2%), bambaranut (57.5%) and groundnut (69.07%) reduction in the phytic acid and tannin content improved the in vitro protein digestibility of the weaning food blends MCBG (86.30 ± 0.53), MCB (77.10 ± 0.2) MCG (73.90 ± 0.04) and MBG (70.80 ± 0.16)

keywords: Maize; Cowpea; Bambaranut; Groundnut

  1. Udensi EA., et al. “Production and evaluation of the nutritional quality of weaning food formulation from roasted millet and Mucuna cochinchinesis”. Sky Journal of Food Science1 (2012): 1-5.
  2. Obimba KC., et al. “Effects of weaning formulae and electrolyte quality of water on rats administer and with contaminated water sours”. African Journal of Biochemistry Research8 (2011): 244-254.
  3. Ijarotimi OS. “Influence of germination and fermentation on chemical composition, protein quality and physical properties of wheat flour (Triticum aestivum)”. Journal of Cereals and Oilseeds3 (2012): 35-47.
  4. Akeredolu IA., et al. “Clinical evaluation of pearl millet conophor weaning mix as supplementary of d of r Nigerian children”. Brazilian Achieve for Biology Technology 48 (2005): 531-536.
  5. Muhimbula HS., et al. “Formulation and sensory evaluation of complementary foods from local, cheap and readily available cereals and legumes in Iringa, Tanzania”. African Journal of Food Science1 (2011): 26-31.
  6. Shiriki D., et al. “Nutritional Evaluation of Complementary Food Formulations from Maize, Soybean and Peanut Fortified with Moringa oleifera Leaf Powder”. Food and Nutrition Sciences5 (2015): 494-500.
  7. Bintu BP., et al. “Studies on the evaluation of the nutritional quality chemical composition and rheological characteristics of a cereal of fortified with legume as a weaning of food blend”. International Journal of Biotechnology and of Food Science1 (2015): 1-9.
  8. Akingbala JO., et al. “A laboratory procedure for the preparation of Ogi, A Nigerian fermented food”. Journal of Food Science5 (1981): 1523-1526.
  9. Theodore I., et al. “Nutritional status of maize fermented meal by fortification with Bambara nut”. African Journal of Food, Agriculture, Nutrition and Development2 (2007): 44-61.
  10. Daves RM. “Some physical properties of groundnut grains”. Research Journal Applied Science Engineering Technology2 (2009): 10- 13.
  11. Association of Official and Analytical Chemist. Official Methods of analysis. Washington DC. 16th Edition 2.44 (2000): 22-33.
  12. Official Methods of Analysis. 18th Edition, Association of Official Analytical Chemists, Arlington (2012): 806-842.
  13. Price LM and Buttler LG. “Rapid visual estimation and spectrophotrometric determination of tannin content of sorghum grains”. Journal of Agricultural and Food Chemistry6 (1977): 1268-1273.
  14. Davies NT and Reid H. “An evaluation of phytate zinc availability form soya based textured-vegetable protein meat substitutes or meat extenders”. British Journal of Nutrition3 (1979): 579-589.
  15. Modu S., et al. “Evaluation of the nutritional value of a composite meal prepared of rm peal millet and cowpea”. Journal of Pure and Applied Science 30 (2010): 1664-1678.
  16. Anigo KM., et al. “Nutrient composition of complementary food gruel formulated from malted cereals, soy beans and groundnut for use in North-Western Nigeria”. African Journal of Food Science3 (2010): 65-75.
  17. Mbata TI., et al. “Evaluation of Mineral content and functional properties of fermented maize (generic and specific) flour blended with bambara groundnut (Vigna susterranean L)”. African Journal of Food Science4 (2009): 107-112.
  18. Mbaeyi IE and Onweluzo JC. “Effect of sprouting and pre-Golatinization on the physic-chemical properties of sorghum-pigeon pea composite blend used for the production of Breaking cereal”. Journal of Tropical Agriculture, Food, Environment and Extension 1 (2010): 8-17.
  19. Nout MJR. “Weaning foods of tropical climates, proceedings of the regional IFS workshop on traditional African of Food Quality and Nutrition, (RIWTAFQNI ’91) Dar-El-Salam, Tanzania, (1991): 23-31.
  20. Oyarekua MA. “Sensory evaluation nutritional quality and antinutritional factors of traditionally co-fermented cereals/cowpea mixture as infant complementary food”. Agriculture and Biology Journal of North America 5 (2011): 950-956.
  21. Malleshie NG., et al. “Development of weaning food of formulation based on malting and roller drying of sorghum and cowpea”. International Journal of Food Science Technology5 (1989): 511-519.
  22. Onilude AA., et al. “Process upgrade and the microbiological nutritional and consumer acceptability of infant weaning food from fermented composite blends of cereals and soybean”. Journal of Food Agriculture and Environment3-4 (2014): 64-68.
  23. Saleh S., et al. “Effects of fortification of rice with banjara beans and sesame on chemical composition mineral elements and vitamin contents of a complementary meal”. EC Nutrition2 (2015): 35-40.
  24. Modu S., et al. “Production and evaluation of chemical composition, tannin in vitro protein digestibility and microbiological studies of fermented local variety of sorgum, fortified with cowpea and groundnut”. Bioscience Research 2 (2012): 99-105.
  25. Tobacco CB., et al. “Analysis of tannin acid in Albino rats”. Journal of Animal Science 52 (2002): 2254-2269.
  26. Hibberd CA., et al. “Nutritive characteristics of Different Varieties of Sorghum and Corn Grains”. Textbook on Cereal McGraw Hill New Delhi (2003): 98-100.
  27. Reddy NK., et al. “Legume-based fermented of foods their preparation and nutritional quality”. Critical Review of Food Science Nutrition 4 (1982): 335-370.
  28. Neelam K and Chauhan BM. “Effect of germination and pure culture fermentation by yeast and lactobacilli on phytic acid and polyphenol content of pearl millet”. Journal of Food Science4 (1990): 1180-1182.
  29. Lopez SD., et al. “Assessment of nutritive value of cereals and legumes strains based on chemical composition and in vitro digestibility (2008).
  30. Sripiya G., et al. “Changes in carbohydrate, free amino acids, organic acids, phytate and HCl extractability of minerals during germination and fermentation of finger millet”. Food Chemistry4 (1997): 345-350.
  31. Nanson NJ and Field ML. “Influence of temperature on the nutrient value of lactic acid fermented corn meal”. Journal of Food Science3 (1984): 958-959.

Modu S., et al. “Studies on the Nutritive Value, Antinutritional Factors and In Vitro Protein Digestibility of Maize Based Food Blend Fortified with Cowpea Bambaranu”. EC Nutrition  10.5 (2017): 199-213.