EC Nutrition

Harmine is one of an active compound can be found in harmala seed. It has a nutritional and pharmacological value, harmine has antimicrobial, antiplasmodial, antifungal, antioxidative, antitumor, antimutagenic, cytotoxic and hallucinogenic properties. Other than having the pharmacological properties, it has been used as an ingredient in many herbal formulations, which are used for the treatment of various diseases. In this study, harmine was isolated from harmal seeds using ethyl acetate and sodium bicarbonate method. An isolated harmine has been identified using thin layer chromatography paper and HPTLC. For harmine purification, pre- parative plate chromatography and designed column chromatography were used. The result of this study revealed that designed column chromatography has several advantages compared to preparative plate Chromatography (PLC) and classical column Chroma- tography. The amount of purified harmine was two times more, saved time and reduces the amount of solvent. The purity of isolated harmine was confirmed using FTIR spectra and c13NMR.

keywords: Harmine; Classical Column Chromatography; New Designed Column Chromatography; Isolation and Purification; C13 NMR

1. Allen J., et al. “The simple b-carboline alkaloids”. Phytochemistry8 (1980): 1573-1582.
2. Callaway J., et al. “Quantitation of N,N-dimethyltryptamine and harmala alkaloids in human plasma after oral dosing with ayahuasca”. Journal of Analytical Toxicology6 (1996): 492- 497.
3. Patel K., et al. “A review on medicinal importance, pharmacological activity and bioanalytical aspects of beta-carboline alkaloid ‘‘Harmine’’. Asian Pacific Journal of Tropical Biomedicine8 (2012): 660-664.
4. Miraj S., et al. “A review of chemical components and pharmacological effects of Melissa officinalis L”. Der Pharmacia Lettre 8.6 (2016): 229-237.
5. Al-shaibani M., et al. “Antitumor and immunomodulatory activities of Pergamum harmala extracts”. Journal of Biotechnology Research Center1 (2013): 11-20.
6. Boeira J., et al. “Genotoxic effects of the alkaloids harman and harmine assessed by comet assay and chromosome aberration test in mammalian cells in vitro”. Journal of Pharmacology and Toxicology6 (2001): 287-294.
7. Herraiz T., et al. “Beta-carboline alkaloids in peganum harmala and inhibition of human monoamine oxidase (MAO)”. Food Chemical Toxicology3 (2010): 839-845.
8. Lacaze A., et al. “Solid-phase extraction and liquid chromatography-mass spectrometry for the determination of free fatty acids in shellfish”. Journal of Chromatograph A1-2 (2007): 51-57.
9. Moreda W., et al. “Gas and liquid chromatography of hydrocarbons in edible vegetable oils”. Journal of Chromatography1-2 (2001): 839-845.
10. Waksmundzka-Hajnos M., et al. “On-line purification and microprpreative separation of contaminated fruit extract by TLC in equilibrium sandwich chamber”. Journal of Planar Chromatography 5 (1992): 169-174.
11. Głowniak K., et al. “The application of zonal thin-layer chromatography to the determination of paclitaxel and deacetylbaccat III in some Taxus species”. Journal of Planar Chromatography5 (1999): 328-335.
12. Hajnos M., et al. “Optimization of the isolation of some toxoids from yew tissues”. Journal of Chromatography 14 (2001): 119-125.
13. Sousek J., et al. “Alkaloids and organic acid content of eight Fumaria species”. Phytochemical Analysis 1 (2009): 6-11.
14. AL-Shahaat N., et al. “Plants and Medicinal Herbs”. Dar AL-Bahaar, Beirut (1986): 140-146.
15. Preston RD. “Hormonal regulation of development molecular aspects of plant hormones”. Cell2 (1981): 573-574.
16. Harrington N. “Harmala alkaloids as bee signaling chemicals”. Journal of Student Research 1 (2012): 23-32.
17. Waksmundzka-Hajnos M., et al. “Strategy for preparative separation of quaternary alkaloids from Chelidonium majus L. by Thinlayer chromatography”. Journal Planar Chromatography4 (2002): 289-293.
18. Shao H., et al. “Main Alkaloids of Peganum harmala L. and Their Different Effects on Dicot and Monocot Crops”. Molecules3 (2013): 2623-2634.
19. Kartal M., et al. “HPLC methods for the analysis of harmal, harmalol, harmaine and harmaline in the seeds of Peganum Harmala”. Journal of Pharmacology Biomedical Analysis2 (2003): 263-296.
20. Aripova S., et al. “Alkaloids of the cultivated species”. Chemistry of Natural Compounds6 (1997): 698-699.
21. Elgubbi H., et al. “Cytotoxicity studies of harmine isolated and purified from Peganum harmala l. seeds”. The Third Symposium on Theories and Applications of Basic and Biosciences 3 (2016): 22-29.
22. Brobst A., et al. “The free base extraction of harmaline from Penganum harmala”. American Journal of Undergraduate Research2-3 (2009): 1-4.
23. Duan A., et al. “Studies on the chemical constituents of Peganum multisectum Maxim II. The alkaloids from seeds and antitumour activity”. Journal of China Pharmaceutical University1 (1998): 21-23.
24. Wang X. “Separation and purification of harmine and harmaline from Peganum harmala using pH-zone-refining counter-current chromatography”. Journal of Separation Science20 (2008): 3543-354.