Research Article Volume 21 Issue 5 - 2026

Effect of Germination on the Mineral and Protein Content of Solojo Flour and Isolates from Two Varieties (DAS and BS) of Nigerian Cultivated Solojo Cowpea (Vigna unguiculata L. Walp)

Olubamike A Adeyoju1, Henry O Chibudike2*, Kayode O Adebowale3, Chinedum E Chibudike4 and Bamidele I OluOwolabi5

1Department of Analytical and Laboratory Management, Federal Institute of Industrial Research, Oshodi, Nigeria
2Department of Chemical, Fiber and Environmental Technology, Federal Institute of Industrial Research, Oshodi, Nigeria
3Department of Chemistry, Industrial Unit, University of Ibadan, Nigeria
4Department of Planning, Technology Transfer and Information Management, Federal Institute of Industrial Research, Oshodi, Nigeria
5Department of Chemistry, Analytical Unit, University of Ibadan, Nigeria

*Corresponding Author: Henry O Chibudike, Department of Chemical, Fiber and Environmental Technology, Federal Institute of Industrial Research, Oshodi, Nigeria.
Received: November 10, 2025; Published: July 31, 2026



Studies on the Mineral Content of Solojo Flour and Protein Isolates from the two varieties (DAS and BS) of Nigeria cultivated solojo cowpeas were conducted to determine their nutritional value. These inorganic elements or minerals were classified into 3 categories, the ultra-trace minerals, which is the third category; the micro elements, also known as the trace minerals is the second category; while the first category are the macro elements, also known as major minerals. Some of the macro-elements are Ca, P, Na and Cl, the second category, micro-elements include, iron, copper, cobalt, potassium, magnesium, iodine, zinc, manganese, molybdenum, F, Cr, Se and S. Results show that the proportion of Sodium (Na) which is ingested into the body in the form of NaCl through food intake maintenance of body pH and to retain water ranged from 728.97 to 253.37 ppm (72.90 to 25.34 mg/100g); 715.24 to 235.45 ppm; 735.28 to 270.37 ppm; 726.59 to 264.35 ppm, for FFDAS, FFBS, DAS and BS respectively with all values of the germinated samples all bellow the control. While FFDAS iron content ranged from 4.25 to 13.50 mg/100g; FFBS ranged from 3.15 to 12.56 mg/100g; DAS ranged from 3.81 to 12.90 mg/100g; BS ranged from 3.42 to 9.40 mg/100g. The values of the germinated flours were all greater than the ungerminated flour. Iron helps to transport oxygen round the body and also helps in red blood cells building and to convert food into needed energy by the body. While Manganese an element that is needed in micro quantity but necessary to convert food into energy, is also crucial for healthy bone and cartilage creation. Results also show that zinc quantity increased as germination proceeded and the values ranged from 38.80 ppm to 230.00 ppm (3.880 mg/100g to 23.00 mg/100g; 0.003880% to 0.0230%); 40.84 to 250.01 ppm; 32.85 to 93.41 ppm; 37.07 to 115.00 ppm, for FFDAS, FFBS, DAS and BS respectively. The Ca content improved significantly (p < 0.05) with sprouting, the value extended from 250.56 ppm to 760.03 ppm (25.056 to 76.00 mg/100g or 0.0251 to 0.0760%); 400.40 to 998.22 ppm; 116.87 to 195.69 ppm; 113.48 to 220.75 ppm, for FFDAS, FFBS, DAS and BS respectively. Zinc element although needed at the micro level in the body, is essential for a strong immune system to keep the body in good health. It is also crucial for the maintenance of a healthy sense of taste and odor, while Calcium is critical for strong bones and teeth, blood coagulation, and muscle tightening and relaxation. Magnesium is needed to build enzyme and antioxidants and also for healthy bones while Potassium is needed to maintain; water balance, muscle movement, and nerve impulses. It functions in conjunction with Na to regulate blood pressure.

 Keywords: Solojo Cowpea; Under-Utilized Legumes; Protein Isolates; BS, DA; Gelation Property, Un-germinated

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Henry O Chibudike., et al. “Effect of Germination on the Mineral and Protein Content of Solojo Flour and Isolates from Two Vari- eties (DAS and BS) of Nigerian Cultivated Solojo Cowpea (Vigna unguiculata L. Walp)”. EC Nutrition 21.5 (2026): 01-11.