EC Veterinary Science

Nine herpes viruses have been identified to infect the family Equidae. EHV-1 is the most important one all over the world. The virus has significant economic importance either in the well-developed countries or the poor ones. So, diagnosis and identification of the causative agent during the outbreaks adding to the structured surveys is crucial to prevent the occurrence and or spreading. Multiple serological techniques like ELISA, complement fixation (CF) and virus neutralization (VN) were routinely used. Recently, molecular characterisation by different PCR types was extensively performed due to its accuracy, easiness, and time saving. Furthermore, the real- time PCR (RT-PCR), enables the quantification of viral loads and differentiation between replicating (lytic), non-replicating or latent virus.

Keywords: EHV-1; Diagnosis; Serological Tools; Molecular; RT-PCR

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