EC Paediatrics

Research Article Volume 12 Issue 3 - 2023

Kumazasa Derivatives Showed an New Anti-Virus Activity

Nobuo Yamaguchi1,2, Marie Izumi2 and Isao Horiuchi2

1Kanazawa Medical University, Japan
2Ishikawa Natural Medicinal Products Research Center, Japan

*Corresponding Author: Nobuo Yamaguchi, Kanazawa Medical University and Ishikawa Natural Medicinal Products Research Center, Japan.
Received: February 08, 2023; Published: February 22, 2023

Background: A novel coronavirus pneumonia (COVID-19) outbreak in Wuhan, China, was caused by the severe acute respiratory syndrome corona virus 2 (SARS-CoV-2), and has now expanded to over 200 countries and territories, infecting more than 2 million people in US and caused over 20 thousand deaths worldwide.

Kumazasa derivatives are reported safety drug to improve anti microorganisms including coronavirus in Japan and its main active ingredient is tricin (4,5,7-trihydroxy-3,5-dimethoxyflavon). The Kumazasa derivatives can suppress the human cytomegalovirus (HCMV) of the DNA virus and the coronavirus pneumonia virus of RNA virus replication in vitro.

Materials and Methods: Human embryonic pulmonary fibroblast (HEL), obtained from human fetal lungs cultivated in 9.6 cm2 shell until confluence with Dulbecco’s Modified Eagle Medium (DMEM) medium was carried out, supplemented with 8% amount of fetal bovine serum (FCS). Towne strain of HCMV has been adsorbed and infected in a variety of infections (MOI) = 1 in HEL cells. After incubation at 37°C for 1 hour, concentrations of 0.1, 1μ, 10 μM of tricin or novel compounds were added and cultivated for 6 days, and infectious HCMV numbers in the culture supernatant were quantified by the plaque method. In the plaque method, HEL cells cultivated to flow into a 24-wells plate were adsorbed on a culture supernatant that was diluted for 1 hour at 37°C 1 hour and then superimposed with DMEM medium with 2% FCS and 0.4% agar for cultivation. After several days (6 to 12 days), the number of plaques displayed under the microscope was counted and the viral load measured.

Results: Comparison of the antiviral effect of GCV, Tricin, Anti-HCMV effects were investigated for several novel compounds found by docking simulation. Here the results that show the most significant effect are shown. The novel compound has been found to exhibit a stronger anti-HCMV effect compared to GCV and Tricin. Next EC-50 values were determined and compared.

Conclusion: The network pharmacological strategy integrated molecular docking to investigate the mechanism of action of AHI against COVID-19. It provides protein targets associated with COVID-19 that can be further tested as therapeutic targets of this safety ingredients.

Keywords: Anti-Virus New CAM; Anti-Cytomegalovirus Agent; CDK9; Tricin; Corona Virus

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Nobuo Yamaguchi., et al. Kumazasa Derivatives Showed an New Anti-Virus Activity. EC Paediatrics 12.3 (2023):36-44.