Research Article Volume 21 Issue 9 - 2025

Induction of Systemic Acquired Resistance against Damping-Off and Stem Rot Diseases of Chickpea Caused by Sclerotinia sclerotiorum

Marwa AM Atwa* and Salama AS El Blasy

Legume and Forage Diseases Research Department, Plant Pathology Research Institute, Agricultural Research Center, Giza, Egypt

*Corresponding Author: Marwa AM Atwa, Legume and Forage Diseases Research Department, Plant Pathology Research Institute, Agricultural Research Center, Giza, Egypt.
Received: July 14, 2025; Published: August 19, 2025



This study investigates the effectiveness of different resistance inducers, Bion (BTH) and salicylic acid (SA) as chemicals, dipotassium phosphate (K2HPO4) as a mineral salt, and ascorbic acid as a vitamin in enhancing chickpea resistance against damping-off and stem rot diseases caused by Sclerotinia sclerotiorum under greenhouse and field conditions. Among the treatments, Bion and SA were the most effective in reducing disease incidence and improving plant survival. In addition, these inducers enhanced growth parameters and increased seed yield. However, the highest levels in mineral content were observed in plants treated with Bion and SA. Notably, there was a marked enhancement in the activities of peroxidase (PO) and polyphenol oxidase (PPO), and an increased accumulation of total phenols compared to the untreated infested control. Dipotassium phosphate and ascorbic acid demonstrated the least effectiveness among the tested treatments, exhibiting moderate efficacy. Furthermore, their performance makes them successful enough to be included as components of integrated disease management programs.

 Keywords: Chickpea; Chemical Inducers; Sclerotinia sclerotiorum

  1. San SH., et al. “Effect of different chickpea genotypes and its biochemical constituents on biological attributes of Helicoverpa armigera (Hübner)”. Legume Research4 (2022): 514-520.
  2. Varshney RK., et al. “Future prospects for chickpea research”. In: The Chickpea Genome. Compendium of Plant Genomes. Springer, Cham (2017): 135-142.
  3. Arriagada O., et al. “A comprehensive review on chickpea (Cicer arietinum) breeding for abiotic stress tolerance and climate change resilience”. International Journal of Molecular Sciences 23.12 (2022): 6794.
  4. Kumar V. “Integrated disease management of sclerotinia rot of chickpea (Cicer arietinum) incited by Sclerotinia sclerotiorum (Lib.) de Bary”. AGBIR5 (2024): 131-132.
  5. Merga B and Haji J. “Economic importance of chickpea: Production, value, and world trade”. Cogent Food and Agriculture1 (2019): 1615718.
  6. Muehlbauer FJ and Sarker A. “Economic importance of chickpea: Production, value, and world trade”. In: Varshney R., Thudi M. and Muehlbauer F. (eds.), The Chickpea Genome, Compendium of Plant Genomes, Springer, Cham (2017).
  7. Wang Z., et al. “Recent advances in mechanisms of plant defense to Sclerotinia sclerotiorum”. Frontiers in Plant Science 10 (2019): 1314.
  8. Antwi-Boasiako A., et al. “Mitigating against Sclerotinia diseases in legume crops: A comprehensive review”. Agronomy12 (2022): 3140.
  9. Boland GJ and Hall R. “Index of plant hosts of Sclerotinia sclerotiorum”. Canadian Journal of Plant Pathology 2 (1994): 93-100.
  10. Adams PB and Ayers WA. “Ecology of Sclerotinia species”. Phytopathology8 (1979): 896-898.
  11. Lane D., et al. “Abiotic conditions governing the myceliogenic germination of Sclerotinia sclerotiorum allowing the basal infection of Brassica napus”. Australasian Plant Pathology 48 (2019): 85-91.
  12. Mwape VW., et al. “Identification of Sclerotinia stem rot resistance quantitative trait loci in a chickpea (Cicer arietinum) recombinant inbred line population”. Functional Plant Biology7 (2022): 634-646.
  13. Mueller DS., et al. “Efficacy of fungicides on Sclerotinia sclerotiorum and their potential for control of sclerotinia stem rot on soybean”. Plant Disease1 (2002): 26-31.
  14. McLaughlin MS., et al. “Why do we need alternative methods for fungal disease management in plants?” Plants (Basel)22 (2023): 3822.
  15. Klessig DF., et al. “Systemic acquired resistance and salicylic acid: Past, present, and future”. Molecular Plant-Microbe Interactions 9 (2018): 871-888.
  16. Thakur M and Sohal BS. “Role of elicitors in inducing resistance in plants against pathogen infection: A review”. ISRN Biochemistry (2013): 762412.
  17. Meena M., et al. “Role of elicitors to initiate the induction of systemic resistance in plants to biotic stress”. Plant Stress 5 (2022): 100103.
  18. Mishra S., et al. “Salicylic acid (SA)-mediated plant immunity against biotic stresses: An insight on molecular components and signaling mechanism”. Plant Stress 11 (2024): 100427.
  19. Naz M., et al. “The past, present, and future of plant activators targeting the salicylic acid signaling pathway”. Genes9 (2024): 1237.
  20. Arslan U. “Evaluation of antifungal activity of mono and dipotassium phosphates against phytopathogenic fungi”. Fresenius Environmental Bulletin3 (2015): 810-816.
  21. Zhou J., et al. “Ascorbic acid in plants: Biosynthesis, regulation and enhancement of stress tolerance”. Frontiers in Plant Science 13 (2022): 837723.
  22. Rangaswami G and Mahadevan A. “An agar blocks technique for isolating soil microorganisms with special reference to pythiaceous fungi”. Science and Culture2 (1999): 85.
  23. Boesewinkel H. “Storage of fungal cultures in water”. Transactions of the British Mycological Society1 (1976): 183-185.
  24. Elsheshtawi M., et al. “Integrated control of white rot disease on beans caused by Sclerotinia sclerotiorum using Contans® and reduced fungicides application”. Saudi Journal of Biological Sciences2 (2017): 405-409.
  25. Willetts HJ and Wong JAL. “The biology of Sclerotinia sclerotiorum, trifolium, and S. minor with emphasis on specific nomenclature”. The Botanical Review 46.2 (1980): 101-165.
  26. Saharan GS and Mehta N. “Sclerotinia diseases of crop plants: Biology, ecology and disease management”. Springer Science & Business Media (2008): 485.
  27. Doyle JJ and Doyle JL. “Isolation of plant DNA from fresh tissue”. Focus1 (1990): 13-15.
  28. White TJ., et al. “Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics”. In: Innis M.A., et al. (eds.), PCR Protocols: A Guide to Methods and Applications. Academic Press, London, UK. (1990): 315-322.
  29. Thompson JD., et al. “CLUSTAL W: Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice”. Nucleic Acids Research 22 (1994): 4673-4680.
  30. Tamura K., et al. “MEGA11: Molecular evolutionary genetics analysis version 11”. Molecular Biology and Evolution 7 (2021): 3022-3027.
  31. Jukes TH. and Cantor CR. “Evolution of protein molecules”. In: Munro H.N. (ed.), Mammalian Protein Metabolism. Academic Press, New York. (1969): 21-132.
  32. Bester MC. “Inoculation techniques and evaluation methodologies for Sclerotinia sclerotiorum head and stem rot in sunflower and soybean”. M.Sc. Thesis, University of the Free State, South Africa (2018): 142.
  33. Cottenie A., et al. “Chemical analysis of plants and soils”. Laboratory of Analytical and Agrochemistry, State University, Ghent, Belgium (1982): 63.
  34. Cottenie A., et al. “Fractionation and determination of trace elements in plants, soils and sediments”. Pure and Applied Chemistry 1 (1980): 45-53.
  35. Chakraborty MR and Chatterjee NC. “Interaction of Trichoderma harzianum with Fusarium solani during its pathogenesis and the associated resistance of the host”. Asian Journal of Experimental Sciences 2 (2007): 351-355.
  36. Sadasivam S and Manickam A. “Biochemical methods”. 2nd edition, New Age International Pvt. Ltd. Publishers and T.N. Agricultural University, Coimbatore, Tamil Nadu, India (1996): 108-110.
  37. Sutha R., et al. “Changes in protein and amino acid composition of tomato due to a tospovirus infection”. Indian Phytopathology2 (1998): 136-139.
  38. Snell FD and Snell CT. “Colorimetric methods of analysis, including some turbidimetric and nephelometric methods”. 3rd edition, Volume III (Organic I), D. Van Nostrand Co. Inc., Princeton, NJ, USA (1953): 606.
  39. Silva F and Azevedo CA. “Principal components analysis in the software Assistat—Statistical Attendance”. In: World Congress on Computers in Agriculture, 7, Orlando. Proceeding, American Society of Agricultural and Biological Engineers (2009).
  40. Bektas Y and Eulgem T. “Synthetic plant defense elicitors”. Frontiers in Plant Science 5 (2015): 804.
  41. Grant M and Lamb C. “Systemic immunity”. Current Opinion in Plant Biology 4 (2006): 414-420.
  42. Spychalski M., et al. “Use of new BTH derivative as supplement or substitute of standard fungicidal program in strawberry cultivation”. Agronomy6 (2021): 1031.
  43. Conrath U., et al. “Priming for enhanced defense”. Annual Review of Phytopathology 53 (2015): 97-119.
  44. Li A., et al. “Action of salicylic acid on plant growth”. Frontiers in Plant Science 13 (2022): 878076.
  45. Faize L and Faize M. “Functional analogues of salicylic acid and their use in crop protection”. Agronomy1 (2018): 5.
  46. Idrees M., et al. “Salicylic acid mitigates salinity stress by improving antioxidant defense system and enhances vincristine and vinblastine alkaloids production in periwinkle (Catharanthus roseus (L.) G. Don)”. Acta Physiologiae Plantarum 33 (2011): 987-999.
  47. Iriti M., et al. “Benzothiadiazole enhances resveratrol and anthocyanin biosynthesis in grapevine, meanwhile improving resistance to Botrytis cinerea”. Journal of Agricultural and Food Chemistry 14 (2004): 4406-4413.
  48. Sarma BK., et al. “Use of non-conventional chemicals as an alternative approach to protect chickpea (Cicer arietinum) from sclerotinia stem rot”. Crop Protection 7 (2007): 1042-1048.
  49. Benhamou N and Bélanger RR. “Benzothiadiazole-mediated induced resistance to Fusarium oxysporumsp. radicis-lycopersici in tomato”. Plant Physiology 118.4 (1998): 1203-1212.
  50. Almagro L., et al. “Class III peroxidases in plant defense reactions”. Journal of Experimental Botany 2 (2009): 377-390.
  51. Mayer AM. “Polyphenol oxidases in plants and fungi: Going places? A review”. Phytochemistry21 (2006): 2318-2331.
  52. Hammerschmidt R. “Phenols and plant-pathogen interactions: The saga continues”. Physiological and Molecular Plant Pathology 3 (2005): 77-78.
  53. Kaur H., et al. “Salicylic acid improves nitrogen fixation, growth, yield and antioxidant defense mechanisms in chickpea genotypes under salt stress”. Journal of Plant Growth Regulation1 (2022): 2034-2047.
  54. Khan MIR., et al. “Alleviation of salt-induced photosynthesis and growth inhibition by salicylic acid involves glycine betaine and ethylene in mungbean (Vigna radiata)”. Plant Physiology and Biochemistry 80 (2014): 67-74.
  55. Wang Z., et al. “Salicylic acid promotes quiescent center cell division through ROS accumulation and down-regulation of PLT1, PLT2, and WOX5”. Journal of Integrative Plant Biology 3 (2021): 583-596.
  56. Ghazanfar M., et al. “Relationship between induced resistance and manganese, zinc, and copper contents of susceptible chickpea cultivars after their inoculation with Ascochyta rabiei”. Pakistan Journal of Phytopathology, 34.2 (2022): 135-145.
  57. Filippi M and Prabhu A. “Relationship between panicle blast severity and mineral nutrient content of plant tissue in upland rice”. Journal of Plant Nutrition8 (1998): 1577-1587.
  58. Ghazanfar M., et al. “Induction of resistance in chickpea (Cicer arietinum) against Ascochyta rabiei by the application of chemicals and plant extracts”. Chilean Journal of Agricultural Research 71.1 (2011): 52-61.
  59. Tripathi R., et al. “Plant mineral nutrition and disease resistance: A significant linkage for sustainable crop protection”. Frontiers in Plant Science 13 (2022): 883970.
  60. Marschner H. “Mineral nutrition of higher plants. 3rd Edition, Academic Press, London (2011): 135-178.
  61. Thongbai P., et al. “Interaction between zinc nutritional status of cereals and Rhizoctonia root rot severity. II. Effect of Zn on disease severity of wheat under controlled conditions”. Plant and Soil 153 (1993): 215-222.
  62. Pilon M., et al. “Copper cofactor delivery in plant cells”. Current Opinion in Plant Biology 3 (2006): 256-263.
  63. Deliopoulos T., et al. “Fungal disease suppression by inorganic salts: A review”. Crop Protection 10 (2010): 1059-1075.
  64. Boubakri H., et al. “Vitamins for enhancing plant resistance”. Planta3 (2016): 529-543.
  65. Ahmed HA., et al. “Induction of resistance in safflower plants against root rot and wilt diseases by certain inducers”. Journal of Phytopathology and Disease Management3 (2017): 23-34.
  66. Wang C., et al. “A comprehensive review on plant ascorbic acid”. Tropical Plants 3 (2024): e042.
  67. Zhang K., et al. “Seed priming with ascorbic acid and spermidine regulated auxin biosynthesis to promote root growth of rice under drought stress”. Frontiers in Plant Science 15 (2024): 1482930.

Marwa AM Atwa and Salama AS El Blasy. “Induction of Systemic Acquired Resistance against Damping-Off and Stem Rot Diseases of Chickpea Caused by Sclerotinia sclerotiorum”. EC Microbiology  21.9 (2025): 01-15.