EC Microbiology

Review Article Volume 19 Issue 1 - 2023

Fluorescent Methods for Test-Analysis of Plankton Fish Eggs

Victoria V Roshchina1*, Valerii A Yashin1, Tatyana N Petrova2 and Vladimir I Maltsev2

1Institute of Cell Biophysics, Federal Research Centre, Pushchino Scientific Centre for Biological Research, Russian Academy of Sciences, Pushchino, Moscow Region, Russia
2TI Vyazemsky Karadag Biological Station, Institute of Southern Seas of the Russian Academy of Sciences, Kurortny, Feodosiya, Cream, Russia

*Corresponding Author: VV Roshchina, Institute of Cell Biophysics, Federal Research Centre, Pushchino Scientific Centre for Biological Research, Russian Academy of Sciences, Pushchino, Moscow Region, Russia.
Received: December 15, 2022;; Published: December 19, 2022



Luminescence microscopy with microspectrofluorimetry have been applied to the study of the fluorescence of fish egg in the ichthyoplankton to identify the state - development or damage. The autofluorescence and fluorescence after histochemical treatment for the biogenic amines (dopamine, histamine and serotonin) as stress indicators of fish eggs from the species of the Black Sea - Engraulis encrasicolus L., Mullus barbatus ponticus Es., Traсhinus draco L., Trachurus mediterraneus ponticus Aleev have been studied. The application of the fluorescent methods may be used for testing of fertilized or damaged eggs at the early stages of development.

 

Keywords: Biogenic Amines; Dopamine; Embryo; Fish Egg; Histamine; Luminescence Microscopy; Microspectrofluorimetry; Serotonin; Shell; Yolk

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VV Roshchina., et al. “Fluorescent Methods for Test-Analysis of Plankton Fish Eggs”. EC Microbiology  19.1 (2023): 25-32.

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