EC Microbiology

Background: As a result of the growth of antibiotic resistance microbes, infectious illnesses have become one of the world's most serious challenges. Medicinal plants are a vast species of plant that are being used to treat a wide variety of infections. To treat bacterial infections, Dodonaea angustifolia (DA) is employed. Dodonaea angustifolia (DA) is used for the treatment of bacterial infections. This study was undertaken from November 2018-April 2019 to determine the active compounds from the leaves of DA and antibacterial effects of fractions.

Methods: Using the agar-well diffusion technique, the antibacterial activity of the solvent fractions (chloroform and ethyl acetate) was investigated against six disease-causing bacteria at varied concentrations. The solvent fractions' minimum inhibitory concentration (MIC) was evaluated using the broth micro-dilution method and tetrazolium chloride.

Result: The sensitive bacterial species were Escherichia coli, Salmonella typhi, and C. freundii. Ethyl acetate and chloroform fractions showed antibacterial activity against the development of harmful bacteria with variable degrees of antibacterial impact. When compared to other bacterial strains, Pseudomonas aeruginosa and Staphylococcus aureus strains were not susceptible to the fraction.

Conclusion: When compared to the chloroform fraction, the ethyl acetate fraction showed substantial antibacterial efficacy against pathogenic bacteria growth. Further research should be carried out to isolate and characterize the bioactive components, as a recommendation.

Keywords: Antibacterial; Dodonaea angustifolia; Test Bacteria; Solvent Fractions

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