EC Microbiology

This research work was done to ascertain the antimicrobial susceptibility of snail mucin on Staphylococcus aureus and Candida albicans. Solution of measured amount of citric acid was used to stimulate the slime production from the snail. Agar in well diffusion method was adopted using Muller Hilton agar. Eight plates of the media was used after inoculum development of the isolates. Ten (10%) and thirty (30%) concentration of both the slime extract and standard drugs (Chloramphenicol and fluconazole) for bacterial and fungal isolates respectively. After 24 hrs and 48 hrs incubation zone of inhibitions were measured. For the 30%, Staphylococcus aureus and Candida albicans gave 17 and 12 mm respectively while chloramphenicol and fluconazole gave 16 and 10 mm respectively. For the 10% concentration, Staphylococcus aureus and Candida albicans gave 3 and 1 mm while chloramphenicol and fluconazole gave 10 and 7 mm respectively. Since the organisms are sensitive to the snail mucin more the standard drug at 30%. The research recommends that the slime can the used to treat infections due to the organisms. It can also be used as additive in cream and probiotics production. These can be done after purification.

 Keywords: Mucin; Purification; Incubation; Sensitive; Inhibition; Zone

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