EC Microbiology

This research work was done to ascertain the bacterial and fungal quality of mixed tapioca popularly called Abaachaa sold at five major bus stops in Ihiala Local government area of Anambra State. The samples were bought and collected from the vendors; mostly women using sterile conical flask. Spread plate technique was done after serial dilution of the samples. The bacterial count on nutrient agar showed 3.8×10¹², 7.7×10¹¹, 3.8×10¹², 6.7×10¹¹, 5.7×10¹¹ cfu/g for Amorka, Uli Center, Total; Post (Okija) bus Stops respectively. Also, bacterial count on MacConkey are 3.5×10¹¹, 7.5×10¹⁰, 3.5×10¹¹, 7.5×10¹⁰, 6.5×10¹⁰ were recorded. For the fungal count; 2.7×10¹⁰, 7.6×10⁹, 3.8×10¹⁰, 5.6×10⁹, 7.6×10⁹ were recorded. Some bacterial species like Bacillus species; Staphylococcus species; Bacillus subtilis; Enterococcus faecalis; Serratia species; Escherichia coli were found. Fugal species like Rhizopus, Penicillium, and Aspergillus species were also isolated. The significance of this study is that it has revealed that the Abaachaa sold at these five bus stops are contaminated and should be eaten with care. Because there was no record of heat treatment in its final preparation both in gravy making and mixing, contamination of the food was noticed. The study recommend that Tapioca (Abaachaa) should be warmed before eaten; though enjoyment of the food is better when taken while it is cold.

 Keywords: Abaachaa; Spread Plate; Serial Dilution; Total-Bacterial; Coliform-Count; Fungal-Count

1. Miriam I and Anthonio HO. “Oil bean salad (Ugbakala or Ugba African salad)”. Food Quality and Safety 5 (2011): 1-9.
2. Nwagu CT., et al. “Nigerian salad recipes-How to make your own”. Journal of Human Sciences3 (2015): 4072-4083.
3. Maky P. “Nigerian salad recipes-How to make your own”. Journal of Human Sciences3 (2013): 4072-4083.
4. Enujiugba VN. “Major fermentative organisms in some Nigerian soup condiments”. Pakistan Journal of Nutrition 8 (2019): 279-283.
5. Miriam I and Anthonio HO. “Oil bean salad (Ugbakala or Ugba African salad)”. Food Quality and Safety 5 (2011): 1-9.
6. Cheesbrough M. “District laboratory practice in tropical countries (3^rd edition)”. Cambridge University Press, India. Part 2 (2010): 133-169.
7. De-Hoog GS., et al. Atlas of clinical Fungi 2^nd (entreat bureau voor 7 Schimmel cultures/universitat Rovira I Virgilli (2017): 1-29.
8. Eni AO., et al. “Microbial quality of fruits and vegetables sold in Sango Ota, Nigeria”. African Journal Food Science5 (2015): 291-296.
9. Enujiugba VN. “Major fermentative organisms in some Nigerian soup condiments”. Pakistan Journal of Nutrition3 (2019): 279-283.
10. Koushki M., et al. “Assessment of the microbial quality of industrial African salads containing meat products”. Current Research in Nutrition and Food Science (2021): 662-670.
11. Oranusi S., et al. “Quality aspects of African salad”. Journal of Emerging Trends in Engineering and Applied Sciences 2 (2013): 287-292.
12. Oranusi S., et al. “Food safety evaluation in boarding schools in Zaria, Nigeria, using the HACCP system”. Scientific Research and Essay10 (2017): 426-433.