EC Clinical and Medical Case Reports

Limosilactobacillus fermentum is found in the gastrointestinal tract of various animals, including human, with potential probiotic properties. A recent study shows that kale juice fermented with L. fermentum EFEL6800 has high kaempferol and quercetin levels, suggesting it a potential candidate for further metabolic engineering for probiotic applications. Kinetic model (KM) is an important tool to guide metabolic engineering but there is no KM of L. fermentum to-date. In this study, we present a whole cell simulatable KM for L. fermentum EFEL6800, IfeTS24, constructed using ab initio approach by identifying enzymes from its genome. The resulting model consists of 931 metabolites, 302 enzymes with corresponding transcriptions and translations, and 853 enzymatic reactions. This can be a baseline model for incorporating other cellular and growth processes, or as a system to examine cellular resource allocations necessary for engineering

 Keywords: Limosilactobacillus fermentum EFEL6800 (lfeTS24); Kinetic Model (KM); Ab Initio

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