EC Paediatrics

Arterial hypertension and diabetes are well known as complex syndromes which lead to multiple malfunctions including fatal vascular contractile abnormalities. Changes in potassium conductance, endothelium-dependent relaxation and myofilament Ca2+ - sensitivity play a crucial role in interplay in contraction/dilation mechanisms of vascular smooth muscle (SM) cells in both hypertension and diabetes. High level of protein kinase C (PKC) activity is appears to be a common feature for hypertension states of different genesis and diabetic vascular disease. We have studied the effectiveness of the RNA interference (RNAi) technique which is able targeting PKC delta-isoform gene and this way to restore vasodilator potential in both spontaneously hypertensive (SHR) and diabetic rats (DR). Experimental design of this study comprised RNAi and patch-clamp techniques in whole-cell configuration, RT-PCR analysis and standard acetylcholine (Ach) test.

1. Kizub I., et al. “Protein kinase C in enhanced vascular tone in diabetes mellitus”. International Journal of Cardiology 2 (2014): 230-242.
2. Novokhatska T., et al. “PKC- δ isozyme gene silencing restores vascular function in diabetic rats”. Journal of Basic and Clinical Physiology and Pharmacology 27 (2014): 1-9.
3. Novokhatska T., et al. “Correction of vascular hypercontractility in spontaneously hypertensive rats using shRNA -induced delta protein kinase C gene silencing”. European Journal of Pharmacology 1-3 (2013): 401-407.