EC Pharmacology and Toxicology

The ethanolic extract of Myxopyrum serratulum (MPSE) exhibits a rich phytochemical profile, including steroids, terpenoids, alkaloids, saponins, phenolics, flavonoids, tannins, and glycosides, which collectively contribute to its notable biological activities. Antioxidant assays such as-Total antioxidant capacity, DPPH free radical scavenging assay and Reducing power assay demonstrate that MPSE possesses moderate free radical scavenging abilities in a dose-dependent manner, likely due to its high phenolic and flavonoid content. In antibacterial evaluations, MPSE shows significant inhibitory effects against both Gram-negative (Pseudomonas aeruginosa) and Gram-positive (Staphylococcus aureus) bacteria, with efficacy increasing at higher concentrations and, in some cases, surpassing standard antibiotics like streptomycin. Cytotoxicity assessments using the MTT assay on L929 fibroblast cells reveal that MPSE is non-toxic, maintaining high cell viability even at elevated concentrations, suggesting its safety for potential therapeutic applications. Spectroscopic analyses, including FTIR and UV-Visible spectroscopy and GC-MS confirm the presence of functional groups and compounds characteristic of bioactive phytochemicals, further supporting the medicinal potential of the extract. Collectively, these findings underscore the promise of Myxopyrum serratulum as a natural source of antioxidant and antimicrobial agent, warranting further research into its pharmacological applications. Collectively, these findings augment the therapeutic potential of M. serratulum, suggesting its relevance for further applications. The uniqueness of this study lies in the investigation of a previously unexplored plant.

 Keywords: Myxopyrum serratulum; Phytochemicals; Antioxidant; Antibacterial; Cytocompatibility

1. Ncube NS., et al. "Assessment techniques of antimicrobial properties of natural compounds of plant origin: current methods and future trends”. African Journal of Biotechnology12 (2008).
2. Sheikh Nilofer., et al. "Phytochemical screening to validate the ethnobotanical importance of root tubers of Dioscorea species of Meghalaya, North East India”. Journal of Medicinal Plants Studies6 (2013): 62-69.
3. Sileshi Dubale., et al. “Phytochemical screening and antimicrobial activity evaluation of selected medicinal plants in Ethiopia”. Journal of Experimental Pharmacology 15 (2023): 51-62.
4. Gopalakrishnan S., et al. "Phytochemical and pharmacognostical studies of the leaves of Myxopyrum serratulum AW Hill”. Journal of Chemical and Pharmaceutical Research 1 (2012): 788-794.
5. Rajalakshmi Karuppasamyand Veerabahu Ramasamy Mohan. "GC-MS analysis of bioactive components of Myxopyrum serratulum AW Hill (Oleaceae)”. International Journal of Pharmaceutical Sciences Review and Research 1 (2016): 30-35.
6. Mensor Luciana L., et al. "Screening of Brazilian plant extracts for antioxidant activity by the use of DPPH free radical method”. Phytotherapy Research2 (2001): 127-130.
7. Prieto Pilar., et al. "Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: specific application to the determination of vitamin E”. Analytical Biochemistry2 (1999): 337-341.
8. Thankamani Vaidyanatha Iyer., et al. "Phytochemical screening and antimicrobial activity of Alstonia scholaris flowers (L) R. BR. Fam: Apocynaceae”. International Journal of Pharmaceutical Research and Development3 (2011): 172-178.
9. Harborne AJ. “Phytochemical methods a guide to modern techniques of plant analysis”. Springer science and business media (1998).
10. Pietta Pier-Giorgio. "Flavonoids as antioxidants”. Journal of Natural Products7 (2000): 1035-1042.
11. Rice-Evans CA., et al. “Structure-antioxidant activity relationships of flavonoids and phenolic acids”. Free Radical Biology and Medicine7 (1996): 933-956.
12. Elavarasan T., et al. “Antioxidant and free radical scavenging activity of the ethanol extract of the stem of Crateva magna (Lour.) DC”. Free Radicals and Antioxidants4 (2012): 20-26.
13. Kaur Charanjit and Harish C Kapoor. "Anti-oxidant activity and total phenolic content of some Asian vegetables”. International Journal of Food Science and Technology2 (2002): 153-161.
14. Oyaizu Makoto. "Studies on products of browning reaction antioxidative activities of products of browning reaction prepared from glucosamine”. The Japanese Journal of Nutrition and Dietetics6 (1986): 307-315.
15. Tiwari P., et al. “Antibacterial activity and physicochemical evaluation of roots of Butea monosperma”. Asian Pacific Journal of Tropical Biomedicine2 (2012): S881-S883.
16. Bibi Sadeer Nabeelah., et al. "Towards the pharmacological validation and phytochemical profiling of the decoction and maceration of Bruguiera gymnorhiza (L.) lam.—a traditionally used medicinal halophyte”. Molecules6 (2022): 2000.
17. Mabry Tom., et al. “The systematic identification of flavonoids”. Springer Science & Business Media (2012).
18. Djeridane A., et al. "Antioxidant activity of some Algerian medicinal plants extracts containing phenolic compounds”. Food Chemistry4 (2006): 654-660.
19. Venugopala Katharigatta., et al. "Review on natural coumarin lead compounds for their pharmacological activity”. BioMed Research International (2013): 963248.
20. Khanam Hena. "Bioactive Benzofuran derivatives: A review”. European Journal of Medicinal Chemistry 97 (2015): 483-504.
21. Dai Jin and Russell J. Mumper. "Plant phenolics: extraction, analysis and their antioxidant and anticancer properties”. Molecules 10 (2010): 7313-7352.
22. Venugopala Katharigatta., et al. "Review on natural coumarin lead compounds for their pharmacological activity”. BioMed Research International (2013): 963248.
23. Torretta Simone., et al. “D-mannose suppresses macrophage IL-1β production”. Nature Communications1 (2020): 6343.
24. Verkerk Ruud., et al. "Glucosinolates in Brassica vegetables: the influence of the food supply chain on intake, bioavailability and human health”. Molecular Nutrition and Food ResearchS2 (2009): S219.
25. Kim Chang H., et al. "Gut microbiota-derived short-chain fatty acids, T cells, and inflammation”. Immune Network 6 (2014): 277-288.