EC Paediatrics

Strychnos camptoneura Gilg and Busse (Loganiaceae), widely used in traditional Congolese medicine, is known for its anti-inflammatory properties. A recent study has reported its effect in Wistar rats using a hydro-ethanolic extract of the leaves and their fractions. This study aims to investigate the anti-inflammatory mechanisms of S. camptoneura. Cell viability was assessed using the MTT colorimetric assay over a concentration range from 0.17 to 5.5 mg/mL for the extract and its fractions. ELISA was used to measure TNF-α, IL-1β, IL-4, and IL-10 levels after inflammation induced in human HaCaT keratinocytes with lipopolysaccharide (LPS). DPPH test was used to assess the anti-radical effect. As a result, we found that, at concentrations of 0.17 and 0.34 mg/mL, cell viability exceeded 60% for the extract and fractions F1, F2, F3, and F4. At these concentrations, the extract and fractions significantly inhibited the secretion of the pro-inflammatory cytokines TNF-α and IL-1β while significantly stimulating the secretion of the anti-inflammatory cytokines IL-4 and IL-10. This effect is more pronounced with the extract and F4 fraction. The extract, F1, F2, F3, and F4, reduce DPPH on the CCM yellow-violet plate and inhibit free radicals with IC50 of 9.03, 18.25, 1.79, 0.39, and 1.5mg/mL, respectively. These results suggest that the fractions and the H.E extract of S. camptoneura leaves act on inflammation by inhibiting pro-inflammatory cytokine secretion against the stimulation of anti-inflammatory cytokines. This effect may be related to the reduction of free radicals.

 Keywords: Strychnos camptoneura; Cytotoxicity; Cytokines; Keratinocytes; Oxidative Stress

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