EC Pharmacology And Toxicology

Introduction: ACPHYX is a recipe of five medicinal plants commonly utilized in Burkina Faso for treating respiratory conditions.

Objectives: The present study objective was to examine the anti-inflammatory and antihistaminic properties of ACPHYX in its hydroethanolic macerated (MHE) and aqueous (MA) forms.

Methods: ACPHYX recipe powder was subjected to hydroalcoholic and aqueous maceration. The antioxidant activity of the extracts obtained was evaluated through DPPH and FRAP tests following phytochemical screening. In vitro anti-inflammatory activity was assessed through pro-inflammatory enzyme inhibition tests (COX1, COX2 and LOX) and in vivo through the carrageenan anti-edema test. The antihistaminic activity was assessed in vivo through the antipruritic test.

Results: The chemical screening revealed the presence of polyphenols, carotenoids, sterols/triterpenes, anthocyanins, saponosides and reducing compounds. The dosage demonstrated a high content of polyphenols and flavonoids in the extracts. The inhibition of DPPH radical by MA found an IC50 13.07 ± 1.18 µg/mL. Its ferric ion reducing capacity was been 1.76 ± 0.2 mmol EAA/ml. The inhibition percentage of LOX have been 33.36 ± 1.09% for MHE and 24.09 ± 0.99% for MA. The COX-1 inhibition percentage were 45.04 ± 4.09% and 35.54 ± 2.34% for MA and MHE. For the COX-2, inhibitions percentage were 13.47 ± 1.07% and 35.54 ± 2.34% respectively for MHE and MA. The MA showed better anti-inflammatory activity in vitro than MHE. The MHE showed better effect antiedema with 79.90% of edema reduction at 600 mg/kg dose. But it better less to reference compound AAS (85.05% of edema reduction at 100 mg/ kg dose). At the same dose 600 mg/kg of MHE, the number of scrapes was reduced at 71.11%. So, a better antihistaminic effect of the extract.

Conclusion: The anti-inflammatory and antipruritic or antihistaminic properties of ACPHYX extracts were attributable to its phytochemical groups. These properties have the potential to alleviate the inflammatory and antihistaminic manifestations associated with respiratory ailments

 Keywords: Anti-Inflammatory; Antihistaminic; ACPHYX Recipe

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