EC Ophthalmology

Research Article Volume 17 Issue 5 - 2026

Modification of a Decellularized Lyophilized Amniotic Membrane for Ophthalmology

Milyudin Evgeny1, Volova LT1, Kuchuk KE2 and Pavlova OV2

1Research Institute of BioTech, Samara State Medical University, Russian Federation
2Samara Regional Clinical Ophthalmological Hospital named after T.I. Eroshevsky, Russian Federation

*Corresponding Author: Milyudin Evgeny, Research Institute of BioTech, 1Samara State Medical University, Russian Federation.
Received: February 12, 2026; Published: September 01, 2026



Introduction: Corneal epithelial regeneration, especially when the basement membrane is damaged, is accompanied by the initiation of angiogenesis and the formation of corneal opacity. To reduce the likelihood of developing pathological changes in the cornea, many authors use amniotic membrane coating. Consequently, the therapeutic effect of amniotic membrane application should result in the absence of newly formed vessels either on the surface or within the cornea, while simultaneously activating proliferation and forming a specific epithelial coating. However, the use of various amniotic membrane preservation methods does not always guarantee the preservation of biologically active substances and the absence of newly formed vessels.

Purpose of the Study: The purpose of this study was to evaluate the feasibility of using decellularized lyophilized amniotic membranes pretreated with glycerol in ophthalmological practice.

Materials and Methods: An experimental study was conducted on two groups of experimental animals. A fragment of decellularized, lyophilized human amniotic membrane was implanted into a subcutaneous pocket in the withers. The first group received a fragment additionally treated with glycerol, while the second group did not. The fragments of decellularized, lyophilized amniotic membrane were subjected to proteome analysis.

Results: A significant difference in the proteomic composition of amniotic membrane treated with glycerol before decellularization and lyophilization was revealed, compared to that not treated with glycerol. Histological examination of the preparations revealed a significant increase in newly formed vessels in animals implanted with a fragment of decellularized, lyophilized amniotic membrane not treated with glycerol.

Conclusion: Therefore, pre-treatment of biomaterial with glycerin and subsequent decellularization using physical methods promotes the preservation of various protein compounds within the tissue structure, which determine biological activity. Specifically, biomaterial not impregnated with glycerin contains a higher protein content, promotes greater cellular infiltration, and activates neoangiogenesis. In contrast, amniotic membrane pre-treated with glycerin does not cause an inflammatory reaction and does not lead to the development of new vessels.

Consequently, in corneal surgery, it is necessary to use lyophilized amniotic membrane pre-treated with glycerin, which has a greater ability to prevent neoangiogenesis.

Keywords: Decellularized Lyophilized Amniotic Membranes; Proteomic Analysis; Morphological Study; Neoangiogenesis

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Milyudin Evgeny., et al. “Modification of a Decellularized Lyophilized Amniotic Membrane for Ophthalmology”. EC Ophthalmology 17.5 (2026): 01-09.

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