EC Gynaecology

Case Report Volume 12 Issue 6 - 2023

Successful Pregnancy Following Injection of Oocyte by Thawed Frozen Sperms Using Testicular Platelet-Rich Plasma (PRP) - Case Report

Rami Alnasser, Mohammad Alghazawi* and Khalil Khalil

British-Syrian IVF Centre, ALRasheed Hospital, Damascus, Syria

*Corresponding Author: Mohammad Alghazawi, Assisted Conception Unit, British-Syrian IVF Centre, ALRasheed Hospital, Damascus, Syria.
Received: April 27, 2023; Published: May 17, 2023

This case report describes a successful pregnancy of a married couple suffering of subfertility for 13 years. The husband diagnosed of having non-obstructed azoospermia after two consecutive failed testicular biopsies. A platelet-rich plasma was injected and a second biopsy was performed after 3 months. The result was a very few immotile sperms. The immotile sperm were frozen. In the ICSI cycle, His wife managed to produce three oocytes after approximately 36 hours of triggering. It was only one mature oocyte was injected with frozen non-motile sperm by mechanical touch method. At 19 hours post injection, the single oocyte was seen fertilized, developed to four-cell embryo on day 2, and transferred at 8-cell stage and graded good on day 3. The pregnancy yielded a positive β-hCG result. The scan performed at six weeks, revealed the presence of one gestation sac with a fetal heartbeat.

Keywords: Platelet-Rich Plasma (PRP); Pregnancy; Azoospermia

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Mohammad Alghazawi., et al. Successful Pregnancy Following Injection of Oocyte by Thawed Frozen Sperms Using Testicular Platelet-Rich Plasma (PRP) - Case Report. EC Gynaecology 12.6 (2023): 05-09.