1Department of Anatomy, Faculty of Medicine, Ardabil Branch, Islamic Azad University, Ardabil, Iran
2Department of Biology, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran
Introduction: Usually, 10 percent of couples in reproductive age are faced with the problem of infertility and need in vitro fertilization treatment. Choosing a suitable egg and sperm in IVF before fertilization is one of the most important steps. Typically, the egg and sperm for fertilization are selected based on appearance. Adult sperms and eggs can ultimately lead embryo to a successful pregnancy. The presence of cumulus cells surrounding the oocyte cytoplasm injection and time are important factors that are discussed in this project.
Methods: Patients with a mean age of 32.7 participated in this project, and after the puncture obtained, eggs were divided into two groups. 94 couples as the "Control group" with the conventional method, and 95 couples as the "Test group" with the proposed method were prepared and punctured. Obtained embryos classified, and embryos transfer into the uterus of the patient was done on the third or fifth day.
Results: 1070 oocytes in control group were containing 721 M2 oocytes (67%), 206 M1 oocytes (19%) and 143 GV oocytes (13%). 927 oocytes (91%) out of 1070 were injected rapidly, and 530 of them (57/1%) became embryos. 1107 oocytes in test group consist of 789 M2 oocytes (71%), 170 M1oocytes (15%) and 145 GV oocytes (13%) which was assaying into groups with 1, 2 or 3 hours postpone in preparing to inject. Finally, 618 (63/7%) out of 970 oocytes (87%) became embryos which is about 6/5% better than conventional method.
Analysis: If oocytes after parrying red blood cells and plasma are incubated for 15 to 30 minutes, obtaining good quality embryos is resulted. The chance of M1 oocytes becoming M2 oocytes at the first hour is high, and generally, obtained embryos of test group (63/7%) in compare with control group embryos (57/1%) have better quality. Also, the quantity of embryos increased about 6/5 percent.
Keywords: In Vitro Oocyte Maturation; Granulosa Cells; ART; Mehr Institute
Maryam Taheri., et al. In Vitro Oocyte Maturation Based on Presence or Absence of Granulosa Cells in the Methods of ART in Mehr Institute. EC Gynaecology 12.7 (2023): 24-30.
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