EC Gynaecology

The application of next-generation sequencing (NGS) methods in studying microbial RNA transcripts provides more understanding about healthy endometrium and uterine dysbiosis related to many gynecological problems. The aim of this opinion article is to assess the benefits and limitations of applying this new knowledge and the future areas for researches. A search of the academic literatures was conducted in various databases and studies published in the last 10 years related to our objectives were included. In conclusion, due to the low- biomass microbial site of the uterus, more studies are essential after establishing standardized methods for analysing these microbial taxa in order to avoid the risk of contaminations. Many studies recommend the use of antibiotics, probiotics or prebiotics as a treatment, yet; there is no evidence based pathway for managing cases with proven uterine dysbiosis.

Keywords: Uterine Microbiomes; Homeostasis; Dysbiosis; Probiotics; Microbial Transplants

1. Molina NM., et al. “New Opportunities for Endometrial Health by Modifying Uterine Microbial Composition: Present or Future?” Biomolecules4 (2020): 593.
2. Dizzell S., et al. “Protective Effect of Probiotic Bacteria and estrogen in Preventing HIV-1-Mediated Impairment of Epithelial Barrier Integrity in Female Genital Tract”. Cells 8 (2019): 1120.
3. Otsuki K and Imai N. “Effects of lactoferrin in 6 patients with refractory bacterial vaginosis”. Biochemistry and Cell Biology 95 (2017): 31-33.
4. Lev-Sagie A., et al. “Vaginal microbiome transplantation in women with intractable bacterial vaginosis”. Nature Medicine 25 (2019): 1500-1504.
5. Romero R., et al. “Evidence that intra- amniotic infections are often the result of an ascending invasion - a molecular microbiological study”. The Journal of Perinatal Medicine 47 (2019): 915-931.
6. Kyono K., et al. “A pilot study and case reports on endometrial microbiota and pregnancy outcome: An analysis using 16S rRNA gene sequencing among IVF patients, and trial therapeutic intervention for dysbiotic endometrium”. Reproductive Medicine and Biology 18 (2019): 72-82.
7. Khodaverdi S., et al. “Beneficial effects of oral lactobacillus on pain severity in women suffering from endometriosis: A pilot placebo-controlled randomized clinical trial”. International Journal of Fertility and Sterility 13 (2019): 178-183.