EC Dental Science

Aim: The aim of this study was observe the influence of fasting and non-fasting in the weight, time of agglutination, and volume of L-PRF and I-PRF.

Materials and Methods: L-PRF and I-PRF were obtain from subjects in fasting and non-fasting.

Results: Fasting state showed better characteristics in volume and weight for L-PRF and I-PRF (p < 0.05) and the time to clot formation in I-PRF was longer in fasting state (p < 0.05). The data showed that volume, weight and time of coagulation for L-PRF and I-PRF is better in fasting state (P < 0.05).

Conclusion: It can be concluded that fasting state promote better characteristics for extraction of L-PRF and I- PRF than non-fasting state.

Clinical Significance: The present study reveals the importance of L-PRF and I-PRF extraction in fasting state for patients that requires oral surgeries because the great characteristics in volume, weight and time of clot coagulation.

Keywords: Fasting; Non-Fasting; Leukocyte Platelet Rich Fibrin; Injectable Platelet Rich Fibrin

1. Karimi K., et al. “The Benefits of Platelet-Rich Fibrin”. Facial Plastic Surgery Clinics of North America3 (2019): 331-340.
2. Kobayashi E., et al. “Comparative release of growth factors from PRP, PRF, and advanced- PRF”. Clinical Oral Investigations9 (2016): 2353-2360.
3. Shah R., et al. “An Update on the Protocols and Biologic Actions of Platelet Rich Fibrin in Dentistry”. The European Journal of Prosthodontics and Restorative Dentistry2 (2017): 64-72.
4. Miron RJ., et al. “Platelet-Rich Fibrin and Soft Tissue Wound Healing: A Systematic Review”. Tissue Engineering Part B, Reviews1 (2017): 83-99.
5. Qing C. “The molecular biology in wound healing and non-healing wound”. Chinese Journal of Traumatology = Zhonghua Chuang Shang Za Zhi4 (2017): 189-193.
6. Marx RE., et al. “Platelet-rich plasma: Growth factor enhancement for bone grafts”. Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics6 (1998): 638-646.
7. Oryan A., et al. “Platelet-rich plasma for bone healing and regeneration”. Expert Opinion on Biological Therapy2 (2016): 213-232.
8. Dohan DM., et al. “Platelet-rich fibrin (PRF): a second-generation platelet concentrate. Part II: platelet-related biologic features”. Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics3 (2006): e45-e50.
9. Dohan DM., et al. “Platelet-rich fibrin (PRF): a second-generation platelet concentrate. Part I: technological concepts and evolution”. Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics3 (2006): e37-e44.
10. Kim TH., et al. “Comparison of platelet-rich plasma (PRP), platelet-rich fibrin (PRF), and concentrated growth factor (CGF) in rabbit-skull defect healing”. Archives of Oral Biology5 (2014): 550-558.
11. Ghanaati S., et al. “Advanced platelet-rich fibrin: a new concept for cell-based tissue engineering by means of inflammatory cells”. The Journal of Oral Implantology6 (2014): 679-689.
12. Fujioka-Kobayashi M., et al. “Optimized Platelet-Rich Fibrin with the Low-Speed Concept: Growth Factor Release, Biocompatibility, and Cellular Response”. Journal of Periodontology1 (2017): 112-121.
13. Mourao CF., et al. “Obtention of injectable platelets rich-fibrin (i-PRF) and its polymerization with bone graft: technical note”. Revista do Colegio Brasileiro de Cirurgioes6 (2015): 421-423.
14. Dohan Ehrenfest DM., et al. “The impact of the centrifuge characteristics and centrifugation protocols on the cells, growth factors, and fibrin architecture of a leukocyte- and platelet- rich fibrin (L-PRF) clot and membrane”. Platelets2 (2018): 171-184.
15. Pieters M., et al. “Diet and haemostasis - a comprehensive overview”. Blood Reviews4 (2015): 231-241.
16. Al-Attas OS., et al. “Association of dietary fatty acids intake with pro-coagulation and inflammation in Saudi Adults”. Asia Pacific Journal of Clinical Nutrition1 (2014): 55-64.
17. Miller GJ. “Dietary fatty acids and the haemostatic system”. Atherosclerosis2 (2005): 213-227.
18. Olas B. “Dietary Supplements with Antiplatelet Activity: A Solution for Everyone?”. Advances in Nutrition1 (2018): 51-57.
19. Yang ZH., et al. “Dietary supplementation with long-chain monounsaturated fatty acid isomers decreases atherosclerosis and alters lipoprotein proteomes in LDLr(-/-) mice”. Atherosclerosis 262 (2017): 31-38.
20. Choukroun JAF., et al. “Une opportunité en paro-implantologie: Le PRF”. Implantodontie 42 (2001): 55-62.
21. Anitua E. “The use of plasma-rich growth factors (PRGF) in oral surgery”. Practical Procedures and Aesthetic Dentistry: PPAD6 (2001): 487-493.
22. Duttaroy AK. “Postprandial activation of hemostatic factors: role of dietary fatty acids”. Prostaglandins, Leukotrienes, and Essential Fatty Acids6 (2005): 381-391.
23. Silveira A. “Postprandial triglycerides and blood coagulation”. Experimental and Clinical Endocrinology and Diabetes: Official Journal, German Society of Endocrinology [and] German Diabetes Association4 (2001): S527-532.
24. Van Der Toorn FA., et al. “Glucose metabolism affects coagulation factors: The NEO study”. Journal of Thrombosis and Haemostasis: JTH11 (2019): 1886-1897.
25. Mutanen M., et al. “Fats, lipids and blood coagulation”. Current Opinion in Lipidology1 (2001): 25-29.
26. Miller GJ. “Effects of diet composition on coagulation pathways”. The American Journal of Clinical Nutrition3 (1998): 542S-545S.