EC Dental Science

Background: Microneedling is a minimally invasive procedure wherein very fine needles are used to induce micro punctures on the skin. Platelet Rich Plasma is a highly promising wound healing agent and hyaluronic acid is an excellent remedy for skin sag. The aim of this study is to analyse the effectiveness of adding PRP and hyaluronic acid to micro-needling therapy, especially in terms of wrinkle and fine line reduction in the face and neck of patients with skin of colour.

Methods: The patients were all of Indian origin. Fifty healthy female patients whose ages were between twenty-five to fifty and who were in different stages of wrinkle and fine line development. Six sessions of micro-needling along with topical application of freshly made PRP mixed with 0.5cc 3.5% hyaluronic acid were done for each patient at an interval of three weeks. Photographs were taken before the beginning of the next session and analysis was done based on the Modified Fitzpatrick Wrinkle Scale. The wrinkle scale reading corresponding to forehead wrinkles, crow’s feet, nasolabial folds and marionette lines were taken into consideration. Micro-needling was done after the application of a topical anaesthetic agent and PRP derived from centrifuging the blood of the patient along with freshly made hyaluronic acid was deposited during micro-needling. The patients were advised to use a gentle cleanser for the face and neck and apply a medical-grade sunscreen with SPF 40 every day without fail.

Results: On analysing the photographs of the fifty patients everyone showed visible improvement in her wrinkle scale right from the second session onwards. Healing was satisfactory no complications developed through the course of the treatment.

Conclusion: Micro-needling combined with PRP and hyaluronic acid proves to be a promising anti ageing regimen providing holistic results by doing damage reversal, maintenance and prevention of accelerated collagen breakdown.

Key Message: Microneedling with topical application of PRP and hyaluronic acid shows considerable wrinkle reduction in Indian skin.

Keywords: Minimally Invasive Treatment; Facial Wrinkle Reduction; Skin of Color; Microneedling; Platelet Rich Plasma; PRP; Hyaluronic Acid

1. GJ F., et al. “Collagen fragmentation promotes oxidative stress and elevates matrix metalloproteinase-1 in fibroblasts in aged human skin”. The American Journal of Pathology 174 (2009): 101-114.
2. BEC NE. “Microneedling in skin of color: A review of uses and efficacy”. Journal of the American Academy of Dermatology2 (2016): 348-355.
3. GL P. “Platelet-Rich Plasma for Skin Rejuvenation: Facts, Fiction, and Pearls for Practice”. Facial Plastic Surgery Clinics of North America3 (2019): 405-411.
4. BG U. “Platelet-rich plasma and hyaluronic acid - an efficient biostimulation method for face rejuvenation”. Journal of Cosmetic Dermatology1 (2017): 112-119.
5. M A., et al. “Efficacy and tolerability of a microneedling device for treating wrinkles on the face”. Journal of Cosmetic Dermatology1 (2023): 206-213.
6. N M., et al. “Microneedling as a Treatment for Acne Scarring: A Systematic Review”. Dermatologic Surgery1 (2020): 86-92.
7. M E-D., et al. “Multiple microneedling sessions for minimally invasive facial rejuvenation: an objective assessment”. International Journal of Dermatology12 (2015): 1361-1369.
8. CE W., et al. “A Single-Center Trial to Evaluate the Efficacy and Tolerability of Four Microneedling Treatments on Fine Lines and Wrinkles of Facial and Neck Skin in Subjects with Fitzpatrick Skin Types I-IV: An Objective Assessment Using Noninvasive Devices and 0.33-mm Microbiopsies”. Aesthetic Surgery Journal11 (2021): NP1603-NP1618.
9. J L and AP S. “Platelet-rich plasma for skin rejuvenation and tissue fill”. Facial Plastic Surgery Clinics of North America4 (2018): 439-446.