EC Cardiology

Numerous methods are known for the microscopic detection of cholesterol and lipid complexes. Nevertheless, histochemical manuals do not mention a simple method of detecting cholesterol and lipid complexes, namely the "non-staining" technique.

Bély and Apáthy’s “non-staining” technique is a simple method to demonstrate cholesterol and lipid crystals in traditionally fixed, paraffin embedded, deparaffinized, unstained tissue sections.

Traditionally processed, but unstained tissue sections examined with polarized light can demonstrate a great variety of different forms of cholesterol and lipid crystals allowing a more precise morphological evaluation.

Keywords: Cholesterol and Lipid Liquid Crystals; Traditional and Non-Staining Technique

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