EC Clinical and Medical Case Reports

Fluoride was determined in 14 food groups of children from 0 to 12 months. Each group, except breast milk, was divided into 10 subgroups, each subgroup with three different batches, resulting in 390 foods analyzed in duplicate, totaling 780, plus 108 breast milk samples, accumulation 888 food samples for children up to 12 months

These groups consist of breast milk, first and second stage formula milk, green and colored vegetables, cereals, potatoes, meats, fish and seafood, legumes, eggs, oils, fruits and water.

The concentrations of fluoride (F^-) in the food were determined by microdiffusion technique and using an ion selective electrode. The concentrations of F^- are breast milk: 0.014 to 0.118 mg/L, formula milk: 0.015 to 0.081 mg/Kg, vegetables: 0.147 to 4.531 mg/Kg, potatoes: 0.140 to 0.880 mg/Kg, fish and shellfish: 0.098 to 5,898 mg/Kg. Legumes: 0.047 and 2.904 mg/Kg. Fruits: 0.090 to 0.406 mg/Kg, and drinking water: 0.574 to 0.643 mg/L. F^- in Subgroups: Luche (Chilean seaweed) 4.531 ± 0.304 mg/Kg and 2.383 ± 0.236 mg/Kg in Cochayuyo (brown seaweed). Coles: 1.091 ± 0.105 mg/Kg. Blanquillo (fish): 1.124 ± 0.137 mg/Kg. Canned sardines and clams: 5,898 ± 1,094 mg/Kg and 4,716 ± 4,379 mg/Kg.

Foods recommended by the Ministry of Health for children under 1 year of age, none of them have high levels of fluoride. All those analyzed (888) have fluoride, some with high concentrations being relevant in total daily intake.

The foods recommended for children from 0 to 12 months do not pose a risk to their oral health due to their fluoride content, with exceptions in foods that are above average.

 Keywords: Fluoride; Food; Analyzed; Children from 0 to 12 Months; Oral Health

1. “Population projections. Base projection. Estimates and projections 2002-2035, commune and urban and rural area. Metropolitan Region” (2017).
2. GL Waldbott. “Acute fluoride intoxication”. Acta Medica Scandinavica 400 (1963): 5-44.
3. PAHO and WHO. “Guiding principles for complementary feeding of the breastfed child”. In: Spanish Pediatrics Association (2018).
4. M Fewtrell., et al. “Complementary Feeding”. Journal of Pediatric Gastroenterology and Nutrition 1 (2017): 119-132.
5. “Growth patterns for nutritional evaluation of children and adolescents, from birth to 19 years of age”. Department of Nutrition and Food (2018).
6. A Bardsen. “Risk periods associated with the development of dental fluorosis in maxillary permanent central incisors: a meta-analysis”. Acta Odontologica Scandinavica5 (1999): 247-256.
7. SM Levy., et al. “Primary tooth fluorosis and fluoride intake during the first year of life”. Community Dentistry and Oral Epidemiology4 (2002): 286-295.
8. PP Hujoel., et al. “Infant formula and enamel fluorosis: a systematic review”. DARE: Quality-assessed Reviews (2009).
9. RW Evans and BW Darvell. “Refining the estimate of the critical period for susceptibility to enamel fluorosis in human maxillary central incisors”. Journal of Public Health Dentistry4 (1995): 238-249.
10. R Yanagida., et al. “Estimation of daily fluoride intake of infants using the microdiffusion method”. Journal of Dental Science1 (2019): 1-6.
11. PE Petersen and H Ogawa. “Prevention of dental caries through the use of fluoride--the WHO approach”. Community Dental Health Journal2 (2016): 66-68.
12. L Singer and R Ophaug. “Total fluoride intake of infants”. Pediatrics 3 (1979): 460-466.
13. JR Heilman., et al. “Fluoride concentrations of infant foods”. Journal of the American Dental Association7 (1997): 857-863.
14. EA Martínez-Mier., et al. “Development of gold standard ion-selective electrode-based methods for fluoride analysis”. Caries Research 1 (2011): 3-12.
15. FV Zohoori and A Maguire. “Are there good reasons for fluoride labelling of food and drink?” British Dental Journal 224 (2018): 215-217.
16. RM Bussell., et al. “Fluoride levels in UK infant milks”. European Archives of Paediatric Dentistry3 (2016): 177-185.
17. CX Harriehausen., et al. “Fluoride intake of infants from formula”. Journal of Clinical Pediatric Dentistry 1 (2019): 34-41.
18. E Koparal., et al. “Fluoride levels in breast milk and infant foods”. Journal of Clinical Pediatric Dentistry4 (2000): 299-302.
19. Y Sener., et al. “Fluoride levels of human plasma and breast milk”. European Journal of Dentistry1 (2007): 21-24.
20. “Sanitary Regulation of Food”. Subsecretary of Public Health Document No. 977/96 D.OF. 13.05.97. (1997).
21. S Van Winkle., et al. “Water and formula fluoride concentrations: significance for infants fed formula”. Pediatric Dentistry Journal4 (1995): 305-310.
22. SNF Mohd Desa S., et al. “A laboratory study of fluoride concentration in infant formulas marketed in Malaysia and estimation of daily intake”. International Food Research Journal5 (2020): 893-902.
23. “Sanitary Regulation of Food”. Document No. 977/96 (D.OF. 13.05.97). TITLE XXVIII. Special Dietary Foods. Paragraph II.- Infant formulas. Articles 493 to 497 (1997).
24. E Vélez-León., et al. “Worldwide variations in fluoride content in beverages for infants”. Children 12 (2023): 1896.
25. N Chandio., et al. “Fluoride content of ready-to-eat infant foods and drinks in Australia”. International Journal of Environmental Research and Public Health21 (2022): 14087.
26. P Cressey. “Dietary fluoride intake for fully formula-fed infants in New Zealand: Impact of formula and water fluoride”. Journal of Public Health Dentistry 4 (2010): 285-291.
27. K Nohno., et al. “Fluoride intake of Japanese infants from infant milk formula”. Caries Research5 (2011): 486-493.
28. LA Dagnaw., et al. “Fluoride content of leafy vegetables, irrigation water, and farmland soil in the rift valley and in non-rift valley areas of Ethiopia”. Fluoride4 (2017): 409-429.
29. N Bhat., et al. “Assessment of Fluoride Concentration of Soil and Vegetables in Vicinity of Zinc Smelter, Debari, Udaipur, Rajasthan”. Journal of Clinical and Diagnostic Research10 (2015): ZC63-ZC66.
30. A Jain., et al. “Estimating concentration of fluoride in edible leaves locally grown around Raipur, Chhattisgarh”. Journal of Indian Association of Public Health Dentistry2 (2017): 177-180.
31. M Maina., et al. “Analysis of Fluoride in Kales (Brassica oleracea) and Tomatoes (Lycopersicum esculentum) from Nakuru County, Kenya”. Africa Environmental Review Journal1 (2020): 35-42.
32. PK Gevera., et al. “Potential fluoride exposure from selected food crops grown in high fluoride soils in the Makueni County, south-eastern Kenya”. Environmental Geochemistry and Health12 (2022): 4703-4717.
33. FSAI Report. “Total Diet Study 2014-2016: Assessment of dietary exposure to fluoride in adults and children in Ireland in 2018”. The Exchange George’s Dock, IFSC. Dublin D01 P2V6.
34. “Feeding guide for children under 2 years old and feeding guide up to adolescence”. Department of Nutrition and Food of the Ministry of Health (2023): 1-145.
35. JA Camargo. “Fluoride toxicity to aquatic organisms: A review”. Chemosphere3 (2003): 251-264.
36. H Al-Adilah., et al. “Iodine and fluorine concentrations in seaweeds of the Arabian gulf identified by morphology and DNA barcodes". Botanica Marina 6 (2020): 509-519.
37. J Itoh., et al. “Elemental analysis of edible plants in natural environment: Trace elements in wild plants”. International Journal of PIXE3 (2007): 119-127.
38. “National Fluoride Database of Selected Beverages and Foods, Release 2”. Nutrient Data Laboratory. Beltsville Human Nutrition Research Center. Agricultural Research Service (2005).
39. JR Jaudenes., et al. “Fluoride risk assessment from consumption of different foods commercialized in a European region”. Applied Sciences18 (2020): 6582.
40. R Havale., et al. “Estimation of fluoride uptake in soil and staple food crops produced in highly fluoridated and non fluoridated regions of Raichur district, Karnataka, India”. Journal of Family Medicine and Primary Care7 (2022): 3546-3552.
41. BD Hong., et al. “Fluoride in soil and plant”. Korean Journal of Agricultural Science4 (2016): 522-536.
42. A Contreras. “Importance of Native Potatoes in Chile”. INIATierra Adentro. Special Genetic Resources (2009): 39-41.
43. D Sawilska-Rautenstrauch., et al. “Fluorine in vegetables and potatoes from the market in Warsaw, Poland”. Roczniki Państwowego Zakładu Higieny3 (1998): 341-346.
44. G Arora and S Bhateja. “Estimating the fluoride concentration in soil and crops grown over it in and around Mathura, Uttar Pradesh, India”. American Journal of Ethnomedicine 1 (2014): 036-041.
45. W Tewarangsri., et al. “Knowledge level and consumption behavior of native plants, meats, and drinking waters with high fluoride concentrations about the relation to the potential health risk of fluoride in Lamphun Province, Thailand: A Case Study”. Sustainability4 (2022): 8701.
46. A Cantoral., et al. “Fluoride content in foods and beverages from Mexico city markets and supermarkets”. Food and Nutrition Bulletin4 (2019): 514-531.
47. PC Calder. “Omega-3 fatty acids and inflammatory processes”. Nutrients3 (2010): 355-374.
48. P Prabhakar and V Hegde. “Estimation of fluoride in freshwater fish and marine water fish”. Journal of Multidisciplinary Dental Research1 (2021): 37-40.
49. F Zohoori and RM Duckworth. “Fluoride: Intake and metabolism, therapeutic and toxicological consequences”. In Molecular, Genetic, and Nutritional Aspects of Major and Trace Minerals. Elsevier (2017): 539-550.
50. CK Lutter., et al. “Complementary feeding of infants and young children 6 to 23 months of age”. Nutrition Reviews8 (2021): 825-846.
51. FR Greer., et al. “The effects of early nutritional interventions on the development of atopic disease in infants and children: the role of maternal dietary restriction, breastfeeding, hydrolyzed formulas, and timing of introduction of allergenic complementary foods”. Pediatrics4 (2019): e20190281.
52. PL Buldini., et al. “State of the art ion chromatographic determination of inorganic ions in food”. Journal of Chromatography A783.1-2 (1997a): 529-548.
53. L La Pera., et al. “Chapter 35 - Inorganic anions in olive oils: application of suppressed ion exchange chromatography (IEC) for the analysis of olive oils produced from de-stoned olives and traditional extraction methods”. Editors: VR. Preedy, RR Watson. Olives and Olive Oil in Health and Disease Prevention. Academic Press (2010): 317-324.
54. R Chaulagain., et al. “Mean fluoride concentration in drinking water sources of a municipality: a descriptive cross-sectional study”. Journal of Nepal Medical Association 255 (2022): 947-951.
55. SA Moimaz., et al. “Fluoride concentration in public water supply: 72 months of analysis”. Brazilian Dental Journal4 (2012): 451-456.
56. “National report on fluoride in drinking water by region year 2021 in Chile”. Department of Oral Health (2022): 1-49.