EC Clinical and Medical Case Reports

Efficacy of Aloe-booster containing ALM (Aqua Life Modifier) green, the official Kampo-plant roots grown regulator, ALM, for the replant failure of Rubia akane, Persicaria tinctoria and Allium cepa, was demonstrated. Inhibition effects of the replant failure with the endophytic fungus of Aloe-booster were shown for R. akane, P. tinctoria and A. cepa. The effects of Aloe-booster in the dried cultivation fields were widely expressed. Potential efficacy of endophytic microbiota, Trichoderma spp. in Aloe vera, against the plant pathogens such as Fusarium spp. as an Aloe-booster, one of bio-stimulants, for replant failure was strongly expected in circulation type-agriculture.

 Keywords: Efficacy; Aloe-Booster; ALM Green; Replant Failure; Rubia akane; Persicaria tinctoria; Allium cepa

1. A Yagi and KM Karen. “Bio-stimulant effects of the fermentation extract by endophytic microbiota from outer leaf layer in Aloe vera”. EC Clinical and Medicinal Case Report6 (2025): 01-05.
2. LP Canellos., et al. “Humic and fulvic acid as bio-stimulants in horticulture”. Scientia Horticulture 196 (2015): 15-27.
3. S Lanzuise., et al. “Combined bio-stimulant applications of Trichoderma with fatty acid mixtures improve bio-stimulant applications of Trichoderma spp. with fatty acid mixtures improve bio-control activity, horticultural crop yield and nutritional quality”. Agronomy 12.2 (2022): 275.
4. MM Rhaman., et al. “Acetic acid: a cheap but chief metabolic regulator abiotic stresses tolerance in plants”. Stress Biology 4 (2024): 34.
5. T Kudo., et al. “Simple and universal function of acetic acid to overcome the drought crisis”. Stress Biology1 (2023): 15.
6. F Zulfiqar., et al. “Bio-stimulants: A sufficiently effective tool for sustainable agriculture in the era of climate change?” Plant Physiology and Biochemistry 211 (2024): 108699.
7. A Yagi., et al. “Application of the fermented extract Aloe vera leaf dry-crushed powders for agricultural behind the concept of DR. Montogomery and A. Bickle: The hidden half of Nature on Organic farming, Case report 1: Efficacy of endophytically formulated extract from twelve crude drugs of Kampo as an official plant roots grown regulator (ALM green) in Japan. Case report 2: Application of the fermented Aloe vera dry-rind powders and ALM green (Aloe-booster) in circulation-type agriculture. Case report 3; Prophylactic role of Aloe vera whole leaf extracted certified by IASC as the purified leaf extract”. EC Clinical and Chemical Case Reports1 (2024): 01-011.
8. F Gracia-Sanchez., et al. “Scientific adventures in bio-stimulation reported the 5^th Bio-stimulant World congress”. Horticulture7 (2022): 665.
9. X Wang., et al. Pharmacognosy Medicine1 (2024): 256-268.
10. W Zeng., et al. “The effective treatment of purpurin on inflammation and adjuvant-induced arthritis”. Molecules1 (2023): 366.
11. Y Wen Qian., et al. “The anti-hypertensive effect of Alizarin is achieved by activating VEGFr2/eNOS pathway, attenuating oxidative stress induced mitochondrial damage and premature senescence”. Life Science 351 (2024): 12286.
12. M Gao., et al. “Simultaneous determination of purpurin, munjistin and mollugin in rat plasma by HPLC-Tandem MS: Application to a pharmacokinetic study after oral administration of Rubia cordifolia extract”. Molecules 21.6 (2016): 717.
13. Q Li., et al. “Chromosome-level genome assembly of the tetraploid medical and natural Persicaria tinctoria”. Scientific Data 11 (2024): 1440.
14. G Kolevski., et al. “Antioxidant in fruits and human medical Research: An overview”. Journal of Hygienic Engineering and Design Review 1 (2012): 271-274.
15. S Fukuda. “Biological functions of Polygonum tinctoria extract”. Kibi International University1-8 (2022).
16. Pei-Yuan Yang., et al. “Targeted discovery of anthranilic acid-based alkaloids with neuroprotective and acetylcholine esterase inhibitory effects from natural indigo plant (　tinctoria) using DeepSAT technology”. Industrial Crops and Products 226 (2025): 120718.
17. M De Tollenaere., et al. “Sunshine in a bottle: Harnessing the power of Persicaria tinctoria extract for Radiant skin and enhanced well-being”. Seifen-Oele-Fette-Washes (SOFW) Journal (2024).
18. P Alejandro., et al. “A comparative study of different strains of Trichoderma under different condition of temperature and pH for the control of Rhizoctonia solani”. Agricultural Science6 (2022): 702-714.
19. Zin NA., et al. “Biological functions of Trichoderma for agricultural applications”. Annales of Agricultural Science 65.2 (2020): 168-178.
20. B Koma. “Assessment of effects of Trichoderma on the root and stem rot disease of Aloe vera plants caused by fungus Rhizoctonia solani”. Phasma Innovation Journal 12.12 (2023): 4199-4201.
21. L-H Wang., et al. “Inhibition effects and mechanisms of the endophytic fungus Trichoderma harzianum LH-7 from Aloe vera”. Ying Yong Sheng Tai Xue Bao 4 (2014): 1130-1136.
22. H Yuef Marting-Parron., et al. “Biocontrol of Fusarium oxysporum by Trichoderma in Aloe vera under green house and field condition”. Mycopath 19.2 (2021): 65-73.
23. S Sasaki., et al. “Optimizing post-harvest processing conditions for Angelica acutiloba roots in Hokkaido: storage temperature and duration”. Journal of Natural Medicines 2 (2025): 328-340.
24. Tucci M., et al. “The beneficial effect of Trichoderma on tomato is modulated by the plant genotype”. Molecular Plant Pathology 12.4 (2011): 341-344.
25. L Al-Madoboly., et al. “Symbiotic effect of Aloe vera juice of Lactobacillus fermentum and helveticus isolates in vitro”. Journal of Gastroenterology and Hepatology Research 6.3 (2017): 2365-2369.
26. H Gafforova. “Study of endophytic bacteria of the medicinal plant Aloe vera”. International Multidisciplinary Research in Academic Science (IMRAS)1 (2025).