EC Clinical and Medical Case Reports

Demystifying the “neuro-immune intersections” in the complex etiopathogenesis of neurological ailments primarily schizophrenia, obsessive compulsive disorder, Alzheimer’s disease and glioblastoma in ethnically disparate susceptible cohorts by immunotherapeutic targeting of Wnt/Frizzled-toll like receptors-autophagy biochemical/metabolic signaling cascades offers fascinating avenues in predictive biomarker development in the Covid-vaccination era [1-4]. CRISPR-Cas genetic engineering technology has emerged as an enigmatic modulator of complex human genetic diseases; precision-medicine based genome editing and detecting specific DNA/RNA sequences to gene expression control warrants future dynamic collaborations for in immuno-inflammatory disease(s)-management in the global Covid-19/Omicron pandemic and Covid-19 vaccination era. At The ability of CRISPR-Cas complexes to be meticulously programmed for targeting particular DNA loci, even when using catalytically inactive dCas-proteins is indeed intriguing; the sophisticated repertoire of naturally derived and bio-engineered dCas-proteins including fusion proteins is a promising toolbox for construction of functional synthetic genetic circuits for critical quantitative understanding of the basic principles governing gene expression regulation and functioning of living organisms [5,6]. In my expert opinion, the disproportionate share of psychosocial distress and neuro-behavioral deficits warrants a robust, evidence-based, pragmatic “public health-bioengineering CRISPR-Cas immunotherapeutic model” for design of pharmacological scaffolds, novel drugs and clinically validated predictive biomarkers for timely management of schizophrenia, obsessive compulsive disorder, Alzheimer’s disease and glioblastoma amongst genetically susceptible at-risk cohorts of asymptomatic vs borderline vs symptomatic heterogeneous subsets of ethnically disparate population-pools of distinct life-styles and socio-economic strata.

1. Pandey S. “Toll-Like Receptors-Autophagy Immuno-Inflammatory Networks as “Molecular Rheostats” in Hypoxic Tumor Microenvironment in Complex Neurological Diseases Primarily Glioblastoma and Spinal Meningioma: Translational Research Perspective with Public Health Impact in Genetically Susceptible Population-Pools in Covid-19/Omicron Pandemic Era”. EC Neurology7 (2022): 01-02.
2. Pandey S. “IL-6 induced upregulation of T-type Ca²⁺ currents and sensitization of DRG nociceptors is attenuated by MNK inhibition: Translational Research Perspective”. Journal of Neurophysiology1 (2020): 305-306.
3. Pandey S and Chandravati. “Targeting Wnt-Frizzled signaling in Cardiovascular Diseases”. Molecular Biology Reports 10 (2013): 6011-6018.
4. Pandey S and Agrawal DK. “Immunobiology of Toll-like Receptors: Emerging trends”. Immunology and Cell Biology4 (2006): 333-341.
5. Kazi TA and Swadesh Ranjan Biswas SR. “CRISPR/dCas system as the modulator of gene expression”. Progress in Molecular Biology and Translational Science 178 (2021): 99-122.
6. Shaytan AK., et al. “From DNA-protein interactions to the genetic circuit design using CRISPR-dCas systems”. Frontiers in Molecular Biosciences 9 (2022): 1070526.