EC Clinical and Medical Case Reports

Research Article Volume 8 Issue 7 - 2025

Biochemical Characterization of Follicular Fluid in Murrah Buffaloes (Bubalus bubalis)

S Mondal1*, Ramanuj Banerjee2, Mamoni Banerjee3, MK Tripathi4, M Samanta5 and VP Varshney6

1ICAR-National Institute of Animal Nutrition and Physiology, Adugodi, Bangalore, Karnataka, India

2Science Counsellor, Embassy of India, Tiergartenstrasse 17, Berlin, Germany

3Rajendra Mishra School of Engineering Entrepreneurship, IIT, Kharagpur, West Bengal, India

4Division of Livestock and Fishery Management, ICAR-Research Complex for Eastern Region, Patna, Bihar, India

5Department of Fish Health Management, ICAR-Central Institute of Freshwater Aquaculture (CIFA), Kausalyaganga, Bhubaneswar, Odisha, India

6ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, India

*Corresponding Author: S Mondal, Principal Scientist, ICAR-National Institute of Animal Nutrition and Physiology, Adugodi, Bangalore, Karnataka, India.
Received: May 22, 2025; Published: June 27, 2025




The aim of the present study was to characterize the composition of buffalo follicular fluid and to correlate the changes with follicular size. Ninety-two ovaries of healthy and non-pregnant adult Murrah buffaloes (n = 46) were collected immediately after slaughter and stored in cold normal saline. Follicular fluid was aspirated from small (3 - 5 mm; n = 124), medium (6 - 9 mm; n = 117) and large (10 - 12 mm, n = 121) ovarian follicles. Follicular fluid samples were analyzed for glucose, cholesterol, creatinine, proteins, triglycerides, bilirubin, urea, acid phosphatase, alkaline phosphatase, GGT (Gamma-glutamyl transferase), progesterone and estradiol. Results showed that follicular fluid concentrations of glucose and cholesterol increased (P < 0.05) from small to large follicles. The concentrations of proteins, triglycerides, bilirubin, urea, acid phosphatase, alkaline phosphatase and GGT decreased as the follicle became larger. The levels of progesterone in follicular fluid increased with the increase in follicular diameter. Lower levels of estradiol 17β were observed in small and large follicles as compared to medium follicles. It can be concluded that the composition of follicular fluid changes from small to large follicles and therefore, may affect the quality of oocyte and granulosa cells of buffalo.

 Keywords: Biochemical; Characterization; Follicular Fluid; Buffalo

  1. FAOSTAT Agriculture Data. Food and Agriculture Organization Statistics, Rome, Italy (2005).
  2. Palta P., et al. “Peripheral plasma inhibin levels in relation to climatic variation and stage of oestrous cycle in buffalo (Bubalus bubalis)”. Theriogenology3 (1997): 989-995.
  3. Mondal S., et al. “Comparison of luteal function between cows and buffaloes during oestrous cycle”. Indian Journal of Dairy Science3 (2002c): 142-144.
  4. Mondal S., et al. “Influence of season on peripheral plasma progesterone in cycling Murrah buffaloes (Bubalus bubalis)”. Buffalo Journal 1 (2004a): 95-100.
  5. Mondal S., et al. “Progesterone and nucleic acid content of buffalo corpus luteum in relation to stages of oestrous cycle”. Indian Journal of Animal Science6 (2004b): 710-712.
  6. Mondal S., et al. “Peripheral plasma inhibin concentrations in relation to expression of estrus in Murrah buffaloes (Bubalus bubalis)”. Indian Journal of Animal Science4 (2003a): 405-407.
  7. Mondal S., et al. “Peripheral plasma progesterone concentration in relation to estrus expression in Murrah buffalo (Bubalus bubalis)”. Indian Journal of Animal Science3 (2003b): 292-293.
  8. Mondal S., et al. “Relationship between peripheral plasma inhibin and FSH concentrations in buffaloes (Bubalus bubalis) and Sahiwal cows (Bos indicus) during estrous cycle”. Tropical Animal Health Production6 (2008a): 403-406.
  9. Mondal S., et al. “Peripheral plasma FSH concentrations in relation to expression of estrus in Murrah buffaloes (Bubalus bubalis)”. Buffalo Bulletin4 (2008b): 258-262.
  10. Colliodal G., et al “Follicular fluid components in reduced ovarian reserve, endometriosis, and idiopathic infertility”. International Journal of Molecular Science 3 (2023): 2589.
  11. Abd Ellah MR., et al. “Ovarian follicular fluid constituents in relation to stage of estrus cycle and size of the follicle in buffalo”. Veterinary World6 (2010): 263-267.
  12. Guerreiro TM., et al. “A metabolomic overview of follicular fluid in cows”. Frontiers in Veterinary Science 5 (2018): 10. 
  13. Ferrazza RDA., et al. “Quantitative proteomic profiling of bovine follicular fluid during follicle development”. Biology of Reproduction6 (2017): 835-849.
  14. Hafez ESE., et al. “Folliculogenesis”. Egg maturation and ovulation chapter 5: In: Reproduction in tarm animal seventh Edition Lippincott Williams and Wilkins. A Wolters Klawer Company Philadelphia, London and New York (2000).
  15. Wise T., et al. “Biochemical analysis of bovine follicular fluid, albumin, total protein, lysosomal enzymes, ions, steroids and ascorbic acid content in relation to follicular size, rank, atresia classification and day of estrous cycle”. Journal of Animal Science4 (2004): 1153-1169.
  16. Mishra OP., et al. “Study on biochemical constituents of caprine ovarian follicular fluid after superovulation”. Asian-Australasian Journal of Animal Sciences12 (2003):1711-1715.
  17. Hung WT., et al. “Biochemical composition of follicular fluid and the effects of culture conditions on the in vitro development of pig oocytes”.Asian-Australasian Journal of Animal Sciences 10 (2002): 1403-1411.
  18. Nandi S., et al. “Biochemical composition of ovine follicular fluid in relation to follicle size”. Development, Growth and Differentiation1 (2007): 61-66.
  19. Thangavel A., et al. “Studies on certain biochemical profile of the buffalo follicular fluid”. The Indian Veterinary Journal 1 (2004): 25-27.
  20. Landau S., et al. “Preovulation follicular status affects the insulin and glucose content of the follicles in high yielding dairy cows”. Animal Reproduction Science3-4 (2000): 181-197.
  21. Leroy JLMR.et al. “Metabolite and ionic composition of follicular fluid from different - sized follicles and their relationship to serum in dairy cows”. Animal Reproduction Science3-4(2004): 201-211.
  22. Kor NM., et al. “Follicular fluid concentrations of biochemical metabolites and trace minerals in relation to ovarian follicle size in dairy cows”. Annual Research and Review in Biology4 (2013): 397-404.
  23. Thakur RS., et al. “Studies on biochemical constituents of caprine follicular fluid”. The Indian Veterinary Journal 80 (2003): 160-162.
  24. Tabatabaei S., et al. “Biochemical composition of blood plasma and follicular fluid in relation to follicular size in buffalo”. Comparative Clinical Pathology5 (2011): 441-445.
  25. Boland NI., et al. “The effect of glucose metabolism on murine follicle development and steroidogenesis in vitro”. Human Reproduction4 (1994):617-623.
  26. Leese JH, et al. “Glucose and lactate in human follicular fluid: concentration and interrelationships”. Human Reproduction 8 (1990): 915-919.
  27. Rabbiee AR., et al. “Relationships among metabolites influencing ovarian function in the dairy cow”. Journal of Dairy Science1(1999): 39-44.
  28. Gosden RG., et al. “Physiological factors underlying the formation of ovarian follicular fluid”. Journal of Reproduction and Fertility2 (1988): 7813-7825.
  29. Balakrishna PP., et al. “Studies on certain factors affecting the in vitro maturation of follicular oocytes in sheep”. (Ph.D. Thesis), Tamil Nadu Veterinary and Animal Sciences University, Chennai, India (1994).
  30. Singh D., et al. “Biochemical and hormonal characterization of follicles from follicular and luteal phase ovaries of goat and sheep”. Indian Journal of Experimental Biology5 (1999): 434-438.
  31. Brantmeier SA., et al. “Concentrations of high-density lipoproteins vary among follicular sizes in the bovine”. Journal of Dairy Science10 (1987): 2145-2149.
  32. Bruchart D. “Lipid absorption and transport in ruminants”. Journal of Dairy Science 12 (1993): 3864-3881.
  33. Puppione DL. “Implications of unique features of blood lipid transport in the lactating cow”. Journal of Dairy Science5 (1977): 651-659.
  34. Bagavandoss P., et al. “Developmental changes in the ovarian follicular basal lamina detected by immunofluorescence and electron microscopy”. Journal of Histochemistry and Cytochemistry5 (1983): 633-640.
  35. Wehrman EE., et al. “Diet-induced hyperlipidimia in cattle modifies the intrafollicular cholesterol environment, modulates ovarian follicular dynamics and hastens the onset of postpartum luteal activity”. Biology of Reproduction3 (1991): 514-522.
  36. Harlow CR., et al. “Gonadotrophic control of human granulose cell glycolysis”. Human Reproduction8 (1987): 649-653.
  37. Grummer RR., et al. “A review of lipoprotein cholesterol metabolism importance to ovarian functions”. Journal of Animal Science12 (1988): 3160-3173.
  38. Moog F. “The physiological significance of the phosphomanoesterases”. Biological Reviews2 (1946):41-59.
  39. Henderson KA., et al. “Acid and alkaline phosphatase in bovine antral follicles”. Journal of Animal Science5 (1990):1363-1369.
  40. Bordoloi PK., et al. “Acid and alkaline fluid cholesterol in goat ovary”. Indian Journal of Animal Research 33 (1999): 144-146.
  41. Parmar AP., et al. “Effect of seasons on enzyme profiles of follicular fluid and blood serum in Surti buffaloes”. Indian Journal of Animal Sciences 61 (1991): 1082-1084.
  42. Short RV. “Steroid present in follicular fluid of cow”. Journal of Endocrinology 23 (1962): 401-407.
  43. Seamark RF., et al. “Steroid hormone production by sheep ovarian follicles cultured in vitro”. Journal of Reproduction and Fertility1 (1974):143-158.

S Mondal., et al. "Biochemical Characterization of Follicular Fluid in Murrah Buffaloes (Bubalus bubalis)." EC Clinical and Medical Case Reports 8.7 (2025): 01-07.

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